【摘要】 目的 建立HPLC法测定盐酸伪麻黄碱的血药浓度。方法:色谱柱为ODS柱(150mm×4.6mm，5μm日本岛津);流动相为0.5%硫酸铵-甲醇(v:v=65∶35);流速1.0ml/min;检测波长为210nm;柱温:室温。结果:本法的标准曲线线性范围为50～1000mg/L;最低检出量为25ng;盐酸伪麻黄碱保留时间为3.5min;平均回收率84.2%;日内RSD小于4.3%;日间RSD小于4.7%。结论:本法检测盐酸伪麻黄碱血药浓度具有快速简便、灵敏准确等优点。

    【关键词】 盐酸伪麻黄碱;血药浓度;反相高效液相色谱

    Determination of pseudoephedrine in human serum by RP-HPLC

    LI Wei-hua，YAN Hui-ping，HUANG Chun，et al.

    Beijing You'an Hospital，Capital University of Medical Sci-ences，Central Lab，Beijing100054，China

    【Abstract】 Objective To develop a HPLC method for the determination of pseudoephedrine in human serum.Methods The analytical column was C18(150mm×4.6mm ......