【摘要】 目的 明确异氟醚减少离体鼠肝缺氧-复氧损伤的作用主要发生在哪一阶段，以及与枯否细胞的关系。方法 将大鼠肝脏取下，置于离体鼠肝灌流仪中，以经95% O2/5% CO2饱和的改良克-林氏碳酸氢盐缓冲液恒压灌流(有氧状态用1.2kPa，缺氧状态用0.2kPa)，维持生理pH值和温度，实时监测灌流液中LDH变化。2MAC异氟醚分别用于缺氧期、复氧期和缺氧-复氧期。用GdCl3选择性抑制枯否细胞活性。结果 (1)2MAC异氟醚用于缺氧-复氧期和单纯用于复氧期均可抑制LDH升高，而单纯用于缺氧期则无明显作用。(2)2MAC异氟醚和(或)GdCl3组均可抑制肝脏缺氧-复氧后LDH升高。结论 异氟醚保护缺氧-复氧肝脏免受损伤的作用主要发挥在复氧期，异氟醚在复氧期发挥保护作用可能与抑制复氧引起的氧自由基爆发有关，其作用与枯否细胞活性密切相关。

    【关键词】 异氟醚；离体鼠肝；缺氧-复氧损伤；枯否细胞

    The effect of isoflurane decrease anoxia-reoxygenation injury in isolated rat liver is related to kupffer cell

    LI Quan,YU Wei-feng,CAO Yun-fei,et al.

    Department of Anesthesiology,Eastern Hepatobiliary Surgical Hospital,Second Military Medical University,Shanghai 200438,China

    【Abstract】 Objective To investigate which stage is the most important and the relationship with kupffer cell,during isoflurane decrease anoxia-reoxygenation injury in isolated rat liver.Methods We established the model of isolated rat liver.It included artificial lung,pump,pressure meter,flow meter,thermostat,humidifier and monitors.Rat livers were harvested and placed in the model.They were flushed with modified Krebs’ buffer,which were saturated with 95% O2 / 5% CO2 and were added with 10 mM glucose and 1% albumin.The perfusion pressures were controlled constantly at 1.2 kPa(oxygenation) or 0.2 kPa(anoxia).PH and temperature were maintained properly,and oxygen pressure or liver flow were measured.2MAC isoflurane were used in the periods of anoxia,reoxygenation or anoxia-reoxygenation separately.GdCl3 was used to inhibit the activation of kupffer cell.Results (1)2MAC isoflurane inhibited LDH release in the periods of both anoxia-reoxygenation and reoxygenation in isolated rat liver,while it was not effective in the period of anoxia.(2)Both 2MAC isoflurane and GdCl3 decreased LDH release obviously after anoxia and reoxygenation in isolated rat liver.Conclusion Reoxygenation stage is the most important during isoflurane decrease anoxia-reoxygenation injury in isolated rat liver.Isoflurane may protect hepatic function by inhibiting oxygen stress outside cells,which is related to kupffer cell. ......