【摘要】 目的 探讨缺血预处理(IPC)对大鼠胰腺缺血再灌注(I/R)损伤的保护作用及其机制。 方法 50只雄性SD大鼠随机分为5组:假手术组(S)仅行剖腹术;对照组(C)胰腺血供阻断60min，再灌注6h，不做预处理;实验1组(E1)预处理，阻断血供5min，再灌注10min;实验2组(E2)预处理，阻断血供10min，再灌注10min;实验3组(E3)预处理，阻断血供15min，再灌注10min。各实验组预处理后，再行胰腺血供阻断60min，再灌注6h。在再灌注完成后取血及胰腺组织标本，检测血清淀粉酶、肿瘤坏死因子-α(TNF-α)和一氧化氮(NO)水平，胰腺细胞凋亡和胰腺组织病理改变。 结果 E1组和E2组与C组比较，血NO浓度明显升高(P<0.01)，TNF-α水平降低(P<0.01)，细胞凋亡指数下降(P<0.01)。 结论 缺血预处理可能通过提高内源性NO合成，抑制TNF-α的产生，减轻细胞凋亡，从而减轻大鼠胰腺I/R损伤。10min缺血，10min再灌注的预处理方式效果较好。

    关键词 缺血 再灌注损伤 胰腺 大鼠

    Effect of ischemic preconditioning on pancreas ischemia reperfusion injury in rats

    Chen Hao，Li Shoubai，Cheng Yunfeng，et al.

    Clinical Center，National Institutes of Health，Bethesda，Maryland20892，USA.

    【Abstract】 Objective To investigate the effect of ischemic preconditioning(IPC)on pancreas ischemia reperfusion(I/R)injury in rats and to define a possible mechanism.Methods Fifty male rats were randomly as-signed to five groups(10rats in each group):Sham group(S):laparotomy only;Control group(C):standard I/R pro-cedure-pancreas blood supply blocked for60-min followed by6h reperfusion;Experimental group1(E1);Experi-mental group2(E2);Experimental group3(E3).IPC was done in all experimental groups before standard I/R proce-dure by block blood supply of pancreas and then reflow was initiated by removal of the clamp.Group E1:5-min is-chemia and10-min reperfusion;Group E2:10-min ischemia and10-min reperfusion;Group E3:15-min is-chemia and10-min reperfusion.Serum amylase ......