【摘要】 目的 建立丹参酮ⅡA的含量测定方法，并比较其干燥前后的转移率。 方法 采用RP-HPLC法，色谱柱为Zorbax SB-C18(4.6mm×250mm);流动相:甲醇-水(3:1);流速:1ml/min;检测波长:270nm;柱温:35℃。 结果 在0.0468～0.234μg范围内峰面积与丹参酮ⅡA浓度呈线性关系，平均回收率为98.22%。 结论 利用HPLC法测定丹参酮ⅡA含量操作简单，结果准确。真空干燥较常温干燥丹参酮ⅡA的转移率有显著提高。

    【关键词】 丹参酮Ⅱ A ;减压干燥;反向高效液相色谱法

    【Abstract】 Objective To establish a simple and sensitive HPLC method for determining TanshinoneⅡA.The contents of TanshinoneⅡA in ethanol extracts which was dried and undried were observed.Methods TanshinoneⅡA was separated on ODS column with a mobile phase of methanol-water(3:1)and detected at270nm with a flow rate of1ml/min.Results The calibration curve was linear within the range of0.0468～0.2340μg(r=0.99996).The average recovery of TanshinoneⅡA was more than98.22%.Conclusion It is a simple and accurate method for the determination of TanshinoneⅡA.The results showed that the best way was to use vacuumwith the less loss of Tanshi-noneⅡA than ordinary pressure.

    【Key words】 tanshinoneⅡ A ;depression dry;HPLC

    丹参为唇形科鼠尾草属植物 ......