【摘要】 目的 研究手术室干罐保存无菌持物钳是否保持无菌状态及其影响因素。 方法 观察不同使用时间及室内不同空气消毒方法对无菌干罐存放持物钳无菌状态的影响，细菌培养采样时使用浸有无菌生理盐水采样液的棉拭子在持物钳尖端部至钳下1/2处(即夹取无菌物品范围)表面反复涂抹数次;同样使用浸有无菌生理盐水采样液的棉拭子在无菌容器的底部及容器下1/2内侧处反复涂抹数次后放入5ml生理盐水试管中送检。 结果 干保存使用中的无菌持物钳、容器存在细菌污染机会，持物钳污染率最高为16.7%，容器污染率最高为23.3%，当室内使用动态空气消毒器时，污染率降低，持物钳使用安全时间得到延长。 结论 手术室无菌持物钳尽量一用一更换，如果采用干保存无菌持物钳，室内应使用动态空气消毒器，并严格掌握使用时限，最好每2h进行更换。

    关键词 无菌持物钳与容器 手术室 无菌操作 医院感染

    Investigation and analysis of axenic holding tongs and containers in operating room

    Xu Quan，Tan Siyuan，Zhang Xiuyun，et al.

    The First People's Hospital of Yancheng，Jiangsu224001.

    【Abstract】 Objective To investigate the axenic state of holding tongs in dry containers and its relative effect whether or not been kept in operating room.Methods Various factors to holding forceps in different using time and fre-quencies and whether or not been kept in air disinfector roomwere observed.Samples were taken from tongs and contain-ers mopping the tip and1/2lower position of tongs and1/2interior position of containers with cotton mop dipped in ster-ile saline solution for several times ......