【摘要】 目的 我们用大鼠异种穿透性角膜移植的动物模型，来研究雷公藤内酯与CsA联合抗移植排斥的效果。 方法 我们用豚鼠角膜对Wistar大鼠实施异种穿透性角膜移植术，分别用雷公藤内酯按100μg/kg体重;CsA注射液按15mg/kg体重;雷公藤内酯和CsA注射液联合用药(100μg/kg+10mg/kg)组抗移植排斥反应。根据Holland等建立的临床观察评分法和免疫组化观察疗效。 结果 (1)临床观察结果表明，CsA和雷公藤内酯联合用药抗排斥作用优于单独给药(P<0.01)。(2)观察外周血T细胞亚群的变化发现CD8 + 细胞的百分数变化联合用药组明显低于CsA或雷公藤单独给药组，CsA组和联合用药组的IL-2R + 细胞百分数变化明显低于雷公藤内酯，它们的差别均有统计学意义(P<0.05)。(3)角膜移植后15天联合用药组植片中淋巴细胞浸润最轻。结论 雷公藤内酯和CsA具有协同抗移植排斥作用。

    【关键词】 雷公藤内酯;环孢素A;移植排斥;角膜移植

    Combination therapy of triptolide and cyclosporine A resist xenocorneal trans-plant rejection

    WANG Jing，SHI Hai-Ming.

    454Hospital of PLA，Nanjing210002，China

    【Abstract】 Objective A xencorneal penetrating keratoplasty model was established in Wistar rat and investigated the effect of anti-rejection by combination therapy under CsA and triptolide.Methods After the corneal of Guinea-pig was transplanted into the eye of Wistar rat，we injected100μg/kg body weight of triptolide，15mg/kg body weight of CsA and100μg/kg+10mg/kg of triptolide and CsA for anti-rejection.The observations on the effect of different anti-rejection agents base the method of Holland and immunochemistry.Results (1)Clinical observation had shown more long-term survival in xenocorneal recipients with combination therapy than triptolide or CsA separat-ing therapy.(2)The changes of percent of CD8 + cell in combination therapy group was less than other therapy groups and percent of IL-2R + cell in combination therapy group and CsA group was less than triptolide group(P<0.05).(3)15days after keratoplasty ......