【摘要】 目的 研究金银花超微饮片的HPLC指纹图谱，考察其有效成分及含量，为金银花的质控提供可靠方法。 方法 色谱柱为大连依利特公司Hypersil BDS C18(4.6mm×200mm，5μm)柱。流动相为甲醇-0.05%磷酸水溶液(9.8∶90.2)，柱温为25℃，流速为1.0ml/min，检测波长为246nm。 结果 建立了金银花超微饮片的HPLC指纹图谱，标定了12个共有峰。 结论 利用HPLC指纹图谱可以较全面地控制金银花超微饮片的内在质量。

    关键词 金银花超微饮片 HPLC指纹图谱 高效液相色谱法

    Studies on HPLC fingerprint of ultramicro Flos Lonicerae

    Cai Guangxian，Li Shunxiang，Jiang Wei

    Hunan Academy of TCM，Changsha410006.

    【Abstract】 Objective To determine the HPLC fingerprint of the ultramicro Flos Lonicerae and to test its po-tent constituents and content.Methods Chromatographic column:Hypersil BDS C18(4.6mm×200mm，5μm).Mo-bile phase:MeOH-0.05%H 3 PO 4 (9.8∶90.2).Column temperature:25℃.Flow rate:1.0ml/min.Wavelength:246nm.Results The fingerprint of ultramicro Flos Lonicerae was established and12common peaks were in the fin-gerprint.Conclusion The methods and data can be used to control the quality of the ultramicro Flos Lonicerae. ......