人脐血间充质干细胞体外诱导分化为类肝细胞
http://www.100md.com
何念海, 赵文利, 王宇明
人脐血间充质干细胞;类肝细胞;分化;白蛋白,何念海,赵文利,王宇明,通讯作者,:,Humanumbilicalcordbloodmesenchymalst
 |
| 第1页 |
参见附件(608KB，5页)。
何念海,赵文利,王宇明,中国人民解放军第三军医大学西南医院全军感染病研究所 重庆市 400038
何念海,男,1965-03-20生,四川省遂宁市人,汉族.第三军医大学医学博士,副教授, 副主任医师,主要从事生物人工肝及相关基础研究.
国家高技术研究发展计划(863)资助项目,No. 2001AA216161
通讯作者:王宇明,400038, 重庆市沙坪坝区高滩岩正街30号,中国人民解放军第三军医大学西南医院全军感染病研究所.
wym417@mail.tmmu.com.cn
收稿日期:2005-04-26 接受日期:2005-06-01
Human umbilical cord bloodmesenchymal stem cells differentiate into functional hepatocypte-likecells in vitro
Nian-Hai He, Wen-Li Zhao, Yu-Ming Wang
Nian-Hai He, Wen-Li Zhao, Yu-Ming Wang, Institute of InfectiousDiseases, Southwest Hospital, the Third Military Medical University,Chongqing 400038, China
Supported by the Fund from 863 High-Technology Program of China,No.2001AA216161.
Correspondence to: Dr. Yu-Ming Wang, Institute of InfectiousDiseases, Southwest Hospital, Third Military Medical University, 30Gaotanyan Zhengjie, Chongqing 400038, China. wym417@mail.tmmu.com.cn
Received: 2005-04-26 Accepted:2005-06-01
Abstract
AIM: To differentiate human umbilicalcord blood mesenchymal stem cells (UCBMSCs) into functional hepatocyte-likecells in vitro, and to identify the molecular biology and functionof hepatocyte-like cells.
METHODS: Human UCBMSCs were isolated and cultured in vitro.Matrigel (10 g/L) was used as matrix, and after pretreated with 2.5 mmol/LAZA for 10-12 h, UCBMSCs were induced in HGF (10 mg/L)+FGF4(10 mg/L)+HGMmedium in vitro. The proliferation of UCBMSCs was detected by MTT.The phenotypes of UCBMSCs were identified by flow cytometry,immunohistochemistry, and reverse transcription polymerase chain reaction(RT-PCR). The level of albumin (ALB) in the supernatant was determinedwith enzyme linked immunosorbent assay (ELISA).
RESULTS: About 150±20adherent cells were obtained in each cord blood unit, and the growth ofthe cells entered log phase 6 days after plantation. The number of UCBMSCsreached 109-1010after 10 generations ......
您现在查看是摘要介绍页，详见PDF附件(608KB，5页)。