CD1小鼠胃肠道快速延迟性整流性钾通道基因的分布
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余 英, 余保平, 汪福群
快速延迟性整流性钾通道基因;原位杂交;免疫组化;逆转录聚合酶链反应;胃肠道;小鼠,余英,余保平,汪福群,通讯作者,:,Expressionofet
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余英, 余保平,汪福群,武汉大学人民医院消化内科 湖北省武汉市 430060
余英, 女,1980-08-27生,湖北省武汉市人,汉族,2003年武汉大学硕士生,主要从事胃肠动力学的基础研究.
通讯作者:余保平,430060, 湖北省武汉市,武汉大学人民医院消化内科. yubaoping62@yahoo.com.cn
电话: 027-68759391
收稿日期:2005-06-06 接受日期:2005-06-13
Expression of ether-a-go-go-related gene in gastrointestinal tract ofnormal CD1 mice
Ying Yu, Bao-Ping Yu, Fu-Qun Wang
Ying Yu, Bao-Ping Yu, Fu-Qun Wang, Department of Gastroenterology,Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China
Correspondence to: Dr. Bao-Ping Yu, Department of Gastroenterology,Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China. yubaoping62@yahoo.com.cn
Received: 2005-06-06 Accepted:2005-06-13
Abstract
AIM: To investigate the expression of ether-a-go-go-related gene (ERG)mRNA and protein and its different distributions in the gastrointestinaltract of CD1 mice.
METHODS: The expression of ERG K+current relatedprotein was examined by immunohistochemistry and the expression of ERGmRNA was detected by reverse transcription polymerase chain reaction (RT-PCR)and in situ hybridization in the tissues of stomach, jejunum, ileumand colon from adult CD1 mice. Then the distribution of ERG was analyzed.
RESULTS: ERG was positively expressed in all the tissues ofstomach, jejunum, ileum and colon. The positive cells mainly distributedin the muscular layer. A small number of positive cells distributed in themucosa and chorion. The levels of ERG expression in stomach and colon weresignificantly higher than those in jejunum and ileum (4.30±0.95, 3.60±0.70vs 2.70±0.82,2.30±1.06; P<0.05).
CONCLUSION: ERG is expressed in the gastrointestinal tract of CD1mice ......
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