HSV1-TK自杀基因系统对小鼠肝癌细胞移植瘤的抑制效应
http://www.100md.com
吴映雅, 杜标炎, 谭宇蕙, 赵 鹏, 王慧峰
单纯疱疹病毒胸苷激酶基因;自杀基因系统;肝癌,吴映雅,谭宇蕙,杜标炎,赵鹏,王慧峰,吴映雅,通讯作者,:,Inhibitoryeffec
 |
| 第1页 |
参见附件(855KB，5页)。
吴映雅,谭宇蕙,广州中医药大学生化教研室 广东省广州市 510405
杜标炎,赵鹏,王慧峰,广州中医药大学病理教研室 广东省广州市 510405
吴映雅,女,1969-03-31生,广东省南海人,汉族,1991年南京大学本科毕业,1997年暨南大学硕士研究生毕业,2003年广州中医药大学在职博士生,讲师, 主要从事中西医结合分子肿瘤学研究.
国家自然科学基金资助项目,No. 30171201
广州市科技计划项目,No. 2002J1-C7041
通讯作者:杜标炎,510405, 广东省广州市机场路12号,广州中医药大学病理教研室. tyuhui@tom.com
电话: 020-36585475
收稿日期:2005-05-13 接受日期:2005-06-15
Inhibitory effect of HSV1-TK suicide gene system induced by retroviruson murine transplanted hepatocarcinoma
Ying-Ya Wu, Biao-Yan Du, Yu-Hui Tan, Peng Zhao, Hui-Feng Wang
Ying-Ya Wu, Yu-Hui Tan, Department of Biochemistry, GuangzhouUniversity of Traditional Chinese Medicine, Guangzhou 510405, GuangdongProvince, China
Biao-Yan Du, Peng Zhao, Hui-Feng Wang, Department of Pathology,Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405,Guangdong Province, China
Supported by National Natural Science Foundation of China, No.30171201, and the Fund from Guangzhou Science and Technology Project, No.2002J1-C7041
Correspondence to: Biao-Yan Du, Department of Pathology, GuangzhouUniversity of Traditional Chinese Medicine, 12 Jichang Road, Guangzhou510405, Guangdong Province, China. tyuhui@Tom.com
Received: 2005-05-13 Accepted: 2005-06-15
Abstract
AIM: To establish the HSV1-tk/GCV tumor suicide system byconstructing a recombinant retroviral vector, and to determine theinhibitory effect of this system on murine transplanted hepetocarcinoma invivo.
METHODS: The HSV-tk cDNA was orientationally cloned into theretroviral vector plasmid (pLXSN) using DNA recombinant technique. Therecombinant plasmid (pLXSN-tk) was mixed with PolyFect Transfectionreagent and was transfected into the packaging cell line PT67. Then, astable virus-producing packaging cell lines was obtained by G418screening, and was transfected into murine hepatocarcinomatous H22 cells ......
您现在查看是摘要介绍页，详见PDF附件(855KB，5页)。