胃癌组织环氧合酶-2表达与幽门螺杆菌cagA+菌株感染
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张 勇，蒋明德，曾维政，徐 辉，王 钊，陈虹彬
CagA+Hpylori感染;环氧合酶-2;原位聚合酶链反应;免疫组织化学,胃癌组织环氧合酶-2表达与幽门螺杆菌cagA+菌株感染,张勇，蒋明德，曾维政，徐辉，王钊，陈虹彬，,通讯作者：,Relati
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张勇，蒋明德，曾维政，徐辉，王钊，陈虹彬，中国人民解放军成都军区总医院消化内科 四川省成都市 610083
通讯作者：张勇，610083，四川省成都市天回镇，中国人民解放军成都军区总医院消化内科. zhangyo1967@163.com
电话：028-86570349
收稿日期：2005-07-19 接受日期：2005-07-28
Relationship between expression of cyclooxygenase-2 and cagA+ Helicobacter pylori infection in gastric carcinoma
Yong Zhang, Ming-De Jiang, Wei-Zheng Zeng, Hui Xu, Zhao Wang, Hong-Bin Chen
Yong Zhang, Ming-De Jiang, Wei-Zheng Zeng, Hui Xu, Zhao Wang, Hong-Bin Chen, Department of Gastroenterology, General Hospital of Chengdu Military Command of Chinese PLA, Chengdu610083, Sichuan Province, China.
Correspondence to: Yong Zhang, Department of Gastroenterology, General Hospital of Chengdu Military Command Chinese PLA, Chengdu 610083, Sichuan Province, China. zhangyo1967@163.com
Received: 2005-07-19 Accepted: 2005-07-28
Abstract
AIM: To explore the relations of cagA+ Helicobacter pylori infection with the expression of cyclooxygenase-2 (COX2) in human gastric carcinogenesis.
METHODS: In situ polymerase chain reaction (PCR) was used to detect the expression of H pylori cagA gene, and immunohistochemical technique was used to detect the expression of COX2 in 56 patients with gastric carcinoma.
RESULTS: The rates of cagA+ H pylori infection and COX2 expression were 55.4% and 71.4%, respectively, in gastric carcinoma. The level of COX2 expression was significantly higher in the gastric carcinoma of Ⅲ-Ⅳ stage than Ⅰ-Ⅱ stage (14/30 vs 5/26, P <0.05), and was also higher in gastric carcinoma with lymph node metastasis than that without lymph node metastasis (4/19 vs 15/37,P <0.01). CagA+ H pylori infection was not correlated with TNM stage and lymph node metastasis in gastric carcinoma (P >0.05). The level of COX2 expression was significantly higher in cagA+ H pylori group than that in cagA- H pylori group (15/31 vs 4/25,P <0.05).
CONCLUSION: CagA+ H pylori infection is not related with the malignant progression of gastric carcinoma, but COX2 expression is involved in the carcinogenesis and malignant progression of gastric carcinoma. CagA+ H pylori infection up-regulates the expression of COX2 in gastric cancer.
Key Words: CagA+ H pylori; Cyclooxygenase-2; In situ polymerase chain reaction; Immunohistochemical technique
Zhang Y, Jiang MD, Zeng WZ, Xu H, Wang Z, Chen HB. Relationship between expression of cyclooxygenase-2 and cagA+ Helicobacter pylori infection in gastric carcinoma. Shijie Huaren Xiaohua Zazhi 2005;13 (17):2141-2144
摘要
目的：探讨幽门螺杆菌(Helicobacter pylori，H pylori)cagA菌株感染及环氧合酶-2 (cyclooxygenase-2，COX2)表达在胃癌发生中的作用及两者的关系.
方法：采用原位聚合酶链反应(PCR)技术及免疫组织化学技术检测56例胃癌手术标本cagA基因及COX2蛋白表达情况.
结果：胃癌组织H pylori cagA菌株感染率为55.4%，COX2在胃癌组织中表达率为71.4%.COX2表达水平在Ⅲ-Ⅳ期胃癌组显著高于Ⅰ-Ⅱ期胃癌组(14/30vs 5/26 ......
张勇，蒋明德，曾维政，徐辉，王钊，陈虹彬，中国人民解放军成都军区总医院消化内科 四川省成都市 610083
通讯作者：张勇，610083，四川省成都市天回镇，中国人民解放军成都军区总医院消化内科. zhangyo1967@163.com
电话：028-86570349
收稿日期：2005-07-19 接受日期：2005-07-28
Relationship between expression of cyclooxygenase-2 and cagA+ Helicobacter pylori infection in gastric carcinoma
Yong Zhang, Ming-De Jiang, Wei-Zheng Zeng, Hui Xu, Zhao Wang, Hong-Bin Chen
Yong Zhang, Ming-De Jiang, Wei-Zheng Zeng, Hui Xu, Zhao Wang, Hong-Bin Chen, Department of Gastroenterology, General Hospital of Chengdu Military Command of Chinese PLA, Chengdu610083, Sichuan Province, China.
Correspondence to: Yong Zhang, Department of Gastroenterology, General Hospital of Chengdu Military Command Chinese PLA, Chengdu 610083, Sichuan Province, China. zhangyo1967@163.com
Received: 2005-07-19 Accepted: 2005-07-28
Abstract
AIM: To explore the relations of cagA+ Helicobacter pylori infection with the expression of cyclooxygenase-2 (COX2) in human gastric carcinogenesis.
METHODS: In situ polymerase chain reaction (PCR) was used to detect the expression of H pylori cagA gene, and immunohistochemical technique was used to detect the expression of COX2 in 56 patients with gastric carcinoma.
RESULTS: The rates of cagA+ H pylori infection and COX2 expression were 55.4% and 71.4%, respectively, in gastric carcinoma. The level of COX2 expression was significantly higher in the gastric carcinoma of Ⅲ-Ⅳ stage than Ⅰ-Ⅱ stage (14/30 vs 5/26, P <0.05), and was also higher in gastric carcinoma with lymph node metastasis than that without lymph node metastasis (4/19 vs 15/37,P <0.01). CagA+ H pylori infection was not correlated with TNM stage and lymph node metastasis in gastric carcinoma (P >0.05). The level of COX2 expression was significantly higher in cagA+ H pylori group than that in cagA- H pylori group (15/31 vs 4/25,P <0.05).
CONCLUSION: CagA+ H pylori infection is not related with the malignant progression of gastric carcinoma, but COX2 expression is involved in the carcinogenesis and malignant progression of gastric carcinoma. CagA+ H pylori infection up-regulates the expression of COX2 in gastric cancer.
Key Words: CagA+ H pylori; Cyclooxygenase-2; In situ polymerase chain reaction; Immunohistochemical technique
Zhang Y, Jiang MD, Zeng WZ, Xu H, Wang Z, Chen HB. Relationship between expression of cyclooxygenase-2 and cagA+ Helicobacter pylori infection in gastric carcinoma. Shijie Huaren Xiaohua Zazhi 2005;13 (17):2141-2144
摘要
目的：探讨幽门螺杆菌(Helicobacter pylori，H pylori)cagA菌株感染及环氧合酶-2 (cyclooxygenase-2，COX2)表达在胃癌发生中的作用及两者的关系.
方法：采用原位聚合酶链反应(PCR)技术及免疫组织化学技术检测56例胃癌手术标本cagA基因及COX2蛋白表达情况.
结果：胃癌组织H pylori cagA菌株感染率为55.4%，COX2在胃癌组织中表达率为71.4%.COX2表达水平在Ⅲ-Ⅳ期胃癌组显著高于Ⅰ-Ⅱ期胃癌组(14/30vs 5/26 ......
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