【摘要】 目的 探讨白血病免疫分型在临床诊断、治疗中的作用。方法 采用免疫荧光标记的方法，用流式细胞仪分析患者血细胞表面各种抗原标记物的表达情况。结果 形态学最初诊断为再生障碍性贫血(AA)的这例病例，经流式细胞术检测出有异常幼稚细胞群体的存在，两次的免疫表型分析显示异常细胞群体由最初的两群细胞克隆演变为一个克隆群体，并带有B细胞系列的抗原标记，临床最终诊断为急性淋巴细胞白血病。目前采用针对B系的化疗方案，疗效较显著。结论 流式细胞术的免疫表型分析对临床的诊断、治疗方案具有重要的参考价值。

    【关键词】 免疫表型；流式细胞术

    【Abstract】 Objective To evaluate the effect of flow cytometric immunophenotyping in clinic diagnosis and therapy.Methods Treated with fluorescein-conjugate antibody, expression of antigen from bone marrow cells was analysed by flow cytometry. Results This case originally diagnosed as AA by morphologic method was discovered to have lots of blasts by flow cytometry. Through two times immunophenotyping analysis, two abnormal colonies developed a colony with B cell antigen. The case was finally diagnosed as ALL and treated with chemotherapy and having a good curative effect. Conclusion Immunophenotyping of flow cytometry have important reference effect on clinic diagnosis and therapy.

    【Key words】 immunophenotyping；flow cytometry

    流式细胞术的免疫表型分析在血液系统疾病领域越来越受到人们的重视 ......