SD大鼠原代颌下腺细胞体外培养的生物学特征研究
颌下腺细胞,,],颌下腺细胞,体外培养,增殖,组织工程,1材料与方法,2结果,3讨论,参考文献
[摘要] 目的:研究SD大鼠颌下腺细胞体外培养的生物学特征,为组织工程化涎腺样组织的体外构建提供有功能的种子细胞。方法:获取新生SD大鼠幼崽颌下腺组织,经酶消化后体外培养,倒置显微镜、电镜下行形态学及免疫细胞化学染色观察。绘制生长曲线,对涎腺细胞的生物特征进行评价。结果:体外培养的颌下腺细胞CK(anti-cytokeratin)及淀粉酶染色阳性,体外增殖活跃。结论:体外培养的颌下腺细胞增殖活跃,保持了腺细胞的生物特征,可作为体外构建组织工程化人工涎腺研究的种子细胞。[关键词] 颌下腺细胞 体外培养 增殖 组织工程
Biological Characteristics of Primary Submandibular Gland Cells from SD Rats EX Vivo.
HUANG Gui-lin, LI Long-jiang, LI Xue-Ying, et al.
Department of Stomatology, Zunyi Medical College,Zunyi, 563003
[Abstract]Objective: To study biological characteristics of primary submandibular gland cells from SD rats, we developed culture system that could obtain plenty of seeding cells for salivary gland organoid tissue engineering. Methods: To harveste submandibular gland from neonatal SD rats, after enzymatically digested we subcultured cells that were observed by phase-contrast and electron microscopy. Immunocytochemical analysis of anti-cytokeratin(CK、CK-1、CK-7、CK-8、CK-14、CK-19), amylase antibody and PAS(periodic acid-schiff) were taken out and the proliferation curve was evaluated ex vivo. Results: Salivary gland cells demonstrated positive staining for cytokeratin and PAS. The cells proliferated actively. Conclusion: The submandibular gland cells maintained its characteristics ex vivo. These functional seeding cells could be used in the tissue engineering study of artificial salivary gland organoids. ......
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