[摘要] 目的：研究SD大鼠颌下腺细胞体外培养的生物学特征，为组织工程化涎腺样组织的体外构建提供有功能的种子细胞。方法：获取新生SD大鼠幼崽颌下腺组织，经酶消化后体外培养，倒置显微镜、电镜下行形态学及免疫细胞化学染色观察。绘制生长曲线，对涎腺细胞的生物特征进行评价。结果：体外培养的颌下腺细胞CK(anti-cytokeratin)及淀粉酶染色阳性，体外增殖活跃。结论：体外培养的颌下腺细胞增殖活跃，保持了腺细胞的生物特征，可作为体外构建组织工程化人工涎腺研究的种子细胞。

    [关键词] 颌下腺细胞 体外培养 增殖 组织工程

    Biological Characteristics of Primary Submandibular Gland Cells from SD Rats EX Vivo.

    HUANG Gui-lin, LI Long-jiang, LI Xue-Ying, et al.

    Department of Stomatology, Zunyi Medical College，Zunyi, 563003

    [Abstract]Objective: To study biological characteristics of primary submandibular gland cells from SD rats, we developed culture system that could obtain plenty of seeding cells for salivary gland organoid tissue engineering. Methods: To harveste submandibular gland from neonatal SD rats, after enzymatically digested we subcultured cells that were observed by phase-contrast and electron microscopy. Immunocytochemical analysis of anti-cytokeratin(CK、CK-1、CK-7、CK-8、CK-14、CK-19), amylase antibody and PAS(periodic acid-schiff) were taken out and the proliferation curve was evaluated ex vivo. Results: Salivary gland cells demonstrated positive staining for cytokeratin and PAS. The cells proliferated actively. Conclusion: The submandibular gland cells maintained its characteristics ex vivo. These functional seeding cells could be used in the tissue engineering study of artificial salivary gland organoids. ......