[摘要] 目的：探讨细胞免疫与复发性阿弗它溃疡(recurrent aphthous ulcer,RAU)发病机制的关系及茶多酚(tea polyphenols ,TP)对RAU的免疫调节作用。方法：用免疫方法建立SD大鼠RAU动物模型，将RAU大鼠随机分成阴性对照组、溃疡糊剂组和3个TP治疗组。用流式细胞仪检测成模前后及用药前后各组大鼠外周血T淋巴细胞亚群CD4+、CD8+细胞数量及比值。结果：实验大鼠溃疡模型成模后外周血CD4+及CD4+/ CD8+较成模前显著降低(P<0.01)，CD8+改变无统计学意义(P>0.05)。茶多酚各治疗组外周血CD4+、CD4+/CD8+回升，与阴性对照组有显著性差异(P<0.05)，茶多酚各治疗组间比较没有显著性差异(P>0.05)。结论：RAU的发生与细胞免疫功能抑制有关，TP对大鼠RAU具有免疫调节作用, 其免疫调节作用与给药方式无关。

    [关键词] 复发性阿弗它溃疡 T淋巴细胞 茶多酚 大鼠

    Experimental Study of Tea Polyphenols on T-Lymphocytes of Recurrent Aphthous Ulcer in Rats.

    LIANG Wen-hong, LIU Jian-guo, GUO Yu-su,et al.

    Department of Oral Medicine, Affiliated Stomatological Hospital of Zunyi Medical College, Zunyi 563003

    [Abstract]Objective: To observe the relationship between cellular immune and the pathogenesis of recurrent aphthous ulcer (RAU) and to evaluate the immune regulation of tea polyphenols(TP)on RAU. Methods: RAU models were established by injecting antigens into SD rats. Five groups, including control group, ulcer cataplasm group and three TP therapeutic groups, were divided randomly. The changes of CD4+ and CD8+ values in blood were measured by flow cytometry (FCM). Results: Compared with normal group, the values of CD4+ and CD4+/CD8+ showed significantly lower level in RAU rats (P<0.01), while CD8+ not(P>0.05). After TP treatment, the values of CD4+ and CD4+/CD8+ increased significantly (P<0.05). It did not show any significant difference among three TP therapeutic groups(P>0.05). Conclusion: The inhibition of cellular immune function may contribute to RAU incidence; TP had the immune regulation on experimental RAU rats, which wasn’t related to the way of administration in this experiment. ......