[摘要] 目的：研究体外培养的人牙囊细胞EGF的表达以及在不同流体静压力作用下对细胞分泌EGF的影响，探讨EGF在牙齿萌出过程中的作用。方法：取年龄为12岁男孩埋藏阻生牙齿的牙囊进行人牙囊细胞培养，将传代的培养细胞应用原位杂交的方法表达EGF，RT-PCR定量分析，分别在50、100 kPa流体静压力作用1 h后RT-PCR方法观察细胞EGF的浓度变化。结果：培养的人牙囊细胞呈梭形，主要为成纤维样细胞，原位杂交染色显示人牙囊细胞中EGF的表达呈阳性，定位于细胞的胞质中，50 kPa流体静压力表达略有增加，100 kPa压力表达明显降低，说明流体静压力在一定力值范围内对人牙囊细胞中有EGF的表达具有空间性。结论：培养的人牙囊细胞具有合成与分泌EGF的能力，流体静压力可以影响体外培养的人牙囊细胞EGF的分泌。

    [关键词] 人牙囊细胞 表皮生长因子 流体静压力

    Expression of Epidermal Growth Factor in Cultured Human Dental Follicle Cells and its Changes under Different Hydrostatic Pressures.

    Jin Zuolin, Lin Zhu, Jiao Guanghai, et al.

    Department of Orthodontics, Stomatological college, The Fourth Military Medical University, Xi’an 710032

    [Abstract]Objective: To investigate the expression and localization of epidermal growth factor (EGF) in cultured human dental follicle cells and the secretion of EGF after incubation under different concentrations of hydrostatic pressure. Methods: the expression and localization of EGF in cultured human dental follicle cells was observed in situ hybridization. RT-PCR was used to quantitate the amount of EGF secreted by the cells after incubation under different hydrostatic pressures: 50、100 kPa. Results: The cultured dental follicle cells showed elongate shape, exhibited fibroblastic characteristics. The expression of EGF showed positive in cells. The EGF surrounding the nucleus was heavily stained. RT-PCR demonstrated an increase in the amount of EGF under 50kPa hydrostatic pressures and decrease under 100kPa. Conclusion: The results indicated that EGF was synthesized and secreted by the dental follicle cells, and the amount of EGF secreted was influenced by different hydrostatic pressures. ......