【摘要】 目的 检测胃癌患者门静脉血细胞角蛋白20(CK20)mRNA,并探讨其临床意义。方法 采用逆转录聚合酶链反应(reverse transcriptase-polymerase chain reaction, RT-PCR)方法检测30例胃癌患者和10例腹腔良性疾病患者术中门静脉血CK20mRNA。结果 胃癌组门静脉血CK20mRNA检出率53.3%(16/30)，对照组门

    静脉血CK20mRNA均为阴性。胃癌患者门静脉血CK20mRNA检出率与临床TNM期别、有无淋巴结转移相关(P<0.05)，并与组织分化及肿瘤浸润深度密切相关(P<0.01)。结论 胃癌血行转移与有无淋巴结转移及TNM期别有关；与组织分化及浸润深度密切相关；有助于临床采取相应的治疗。

    【关键词】 胃肿瘤;逆转录聚合酶链反应;细胞角蛋白20；转移

    Detection of CK20mRNA in portal venous blood with gastric cancer and its clinical implication

    XIANG He-ping, ZHANG Chang-le.

    Department of Surgery, The Affiliated Hospital of Anhui Medical University, Anhui 230000,China

    【Abstract】 Objective To detect Cytokeratin20(CK20) mRNA in portal venous blood from patients with gastric cancer and to study its clinical significance. Methods Reverse transcriptase-polymerase chain reaction(RT-PCR) was used to detect the CK20mRNA in portal venous blood from 30 patients with gastric cancer and 10 patients with benign disease who underwent laparotomy served as controls.Results The positive rate of CK20mRNA in portal venous blood

    was 53.3%, none of 10 patients with benign disease was positive. The positive rate of CK20mRNA in portal venous blood was correlated to the TNM classification and lymph node metastasis(P<0.05) ......