[摘要] 目的：检测骨髓基质细胞系Kusa-A1成骨分化过程中Notch信号相关分子的表达变化，分析Notch信号途径在成骨细胞分化和骨形成中的可能作用。方法：培养小鼠Kusa-A1细胞，在常规培养和诱导培养(添加维生素C和β磷酸甘油)条件下用Real-time PCR方法分别检测Delta1、Jagged1、Notch1、CBF1、HES1基因的表达变化。同时也检测了成骨标志基因骨桥蛋白(OPN)的表达变化。结果：常规培养下Kusa-A1细胞汇片后0~5d，OPN表达有轻微下调，Notch相关分子表达维持不变或有轻微下调。诱导培养条件下细胞汇片后0~5d，OPN表达上调，而Delta1、Jagged1、Notch1、HES1表达大幅度下调，惟有CBF1表达有轻度上调趋势。结论：Notch信号分子表达与Kusa-A1细胞成骨分化负相关，Notch信号对细胞成骨分化可能有抑制作用，而CBF1则可能对成骨细胞分化有正向调节作用。

    [关键词] Notch相关基因 表达 骨髓基质细胞Kusa-A1 成骨分化

    Expression of Notch-related Genes in the Differentiation of Mesenchymal Progenitor Cell, Kusa-A1.

    WANG Sheng-Chao, Kawashima Nobuyuki，Sakamoto Kei, et al.

    Department of Endodontics, College of Stomatology, Fourth Military Medical University, Xi’an 710032

    [Abstract]Objective: To investigate the expression of Notch-related genes in mouse mesenchymal progenitor cell Kusa-A1 and analyze the possible role of Notch pathway in osteogenic differentiation. Methods: Kusa-A1 cells were cultured under normal or mineralization-inducing condition. Total RNA was isolated at day 0, 1, 3, 5 after confluence. Real-time PCR was used to detect the expression of Delta1, Jagged1, Notch1, CBF1, HES1 genes. Expression of osteopontin (OPN) was also examined. Results: Under normal culture condition, OPN expression was slightly down-regulated and level of Notch-related genes remained unchanged or showed slight down-regulation. After the addition of L-ascorbic acid-2-phosphate and β-glycerophosphate, known to induce differentiation in osteogenic cells, OPN expression was up-regulated but expression of Delta1, Jagged1, Notch1 and HES1 all showed striking down-regulation. Interestingly, CBF1 expression exhibited slight up-regulation. Conclusion: Notch pathway is involved and may exert a suppression role in the differentiation of osteogenic cells. However, CBF1, transcriptional mediator of Notch signaling, may have a positive effect on osteogenic differentiation. ......