[摘要] 目的：研究纤维粘连蛋白基因ED-B片段在涎腺正常组织及其良、恶性肿瘤组织中的表达情况，探讨其与涎腺良、恶性肿瘤发生、发展的关系。方法：分别提取涎腺正常组织及其良、恶性肿瘤组织的总RNA，RT-PCR法扩增ED-B片段，重组于pGEM-T easy 载体, 酶切制备成模板，体外逆转录方法合成cRNA探针，通过RT-PCR和原位杂交方法分别检测ED-B mRNA的表达情况。结果：ED-B-在正常涎腺及其良、恶性肿瘤组织中均呈高表达；ED-B+在正常腺体组织中不表达，而在良性肿瘤中有表达，且在恶性肿瘤中其阳性表达程度增高。结论：随着涎腺向肿瘤、恶性肿瘤方向的转化，FN基因ED-B剪切片段的表达逐渐增强。

    [关键词] 涎腺 肿瘤 纤维粘连蛋白 剪切片段 基因表达

    The Expression of ED-B Isoform of Fibronectin gene in Oral salivary gland tumors.

    WANG Hong-lin, ZHAO Huan-ying, LI Cui-ying.

    Department of Stomatology , Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100032

    [Abstract]Objective: The aim of this study was to investigate the expression of ED-B isoform of fibronectin in normal, benign, and malignant tissues of salivary gland, and find the relation ship of ED-B and generation, development and matastasis of salivary gland tumors. Methods： Total RNA was extracted from normal, benign and malignant tissues, ED-B DNA bands were obtained by RT-PCR. The fragments of cDNA were cloned into the pGEM-T easy vector. The sense and antisense templets were made by digestion of the plasmid pGEM-T easy of ED-B with restriction enzymes. The cRNA probes were constructed from the templets by transcription in vitro. Expression of ED-A-, ED-B- and ED-B+ mRNA in different tissues were analyzed by in situ hybridization. Results： The expression of ED-B- was found in all the cases. The expression of ED-B+ had been found to be absent in normal tissues, and was much greater in malignant tissues than those in benign tissues. Conclusions： The expression of ED-B of fibronectin gene increased as with salivary tumors transforming to malignant. ......