树突状细胞在黏膜免疫模型鼠体内的分布及趋化因子的表达
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谢遵江，贺业春，贾立敏，刘 颖，刘 丽
树突状细胞;趋化因子;黏膜免疫鼠模型,谢遵江，贺业春，贾立敏，刘颖，刘丽，,谢遵江,通讯作者：,收稿日期：,接受日期：,Distribution
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谢遵江，贺业春，贾立敏，刘颖，刘丽，哈尔滨医科大学解剖学教研室 黑龙江省哈尔滨市 150086
谢遵江, 男, 1964-02-19生, 黑龙江省哈尔滨市人, 汉族, 2004年人体解剖学博士, 教授, 主要从事肠道黏膜免疫研究.
黑龙江省自然科学基金资助项目，No. D0234
通讯作者：谢遵江, 150086, 黑龙江省哈尔滨市, 哈尔滨医科大学解剖学教研室. xiezj555@hotmail.com
电话：0451-86674508-8001
收稿日期：2005-07-28 接受日期：2005-08-03
Distribution of dendritic cells and expression of chemokines in mouse models of mucosal immune in vivo
Zun-Jiang Xie, Ye-Chun He, Li-Min Jia, Ying Liu, Li Liu
Zun-Jiang Xie, Ye-Chun He, Li-Min Jia, Ying Liu, Li Liu, Department of Anatomy, Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Supported by Natural Science Foundation of Heilongjiang Province, No. D0234
Correspondence to: Zun-Jiang Xie, Department of Anatomy, Harbin Medical University, Harbin 150086, Heilongjiang Province, China. xiezj555@hotmail.com
Received: 2005-07-28 Accepted: 2005-08-03
Abstract
AIM: To investigate the migration, distribution and morphological characteristics of dendritic cells (DCs) and the expression of chemokines in the mouse models of mucosal immune.
METHODS: The distribution and morphological characteristics of DCs in the mouse models were observed by light microscopy, immunohistochemical method, and in situ hybridization staining. The expression of S-100 protein, monocyte chemotactic protein-1(MCP-1), regulated upon activation, normal T cell expressed and secreted chemokine (Rantes), macrophage inflammatory protein-1β(MIP-1β) mRNA and C-C chemokine receptor 7(CCR7) mRNA were analyzed.
RESULTS: The in vivo DCs were mainly located in the mesenteric lymph node(MLN), Peyer’s Patch(PP), and tissues of gut in the mouse model of mucosal immune. The number of DCs was significantly larger in the model group than that in the controls(35.32 ± 3.17 vs 15.03 ± 1.81, 29.14 ± 2.03 vs 11.35 ± 0.97, 27.96 ± 1.97 vs 9.86 ± 0.82, P <0.01). The expression of MCP-1, Rantes, MIP-1βmRNA and CCR7 mRNA in the mucosal immune mice were significantly increased, and their number and area densities were markedly higher than those the controls ......
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