便秘型肠易激综合征结肠黏膜组织蛋白质组双向凝胶电泳分析
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彭丽华，杨云生，孙 刚，王巍峰
结肠疾病; 便秘; 蛋白质组; 双向凝胶电泳; 肠易激综合征,马振海,戴冬秋,徐惠绵，,马振海
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马振海, 戴冬秋,徐惠绵，中国医科大学附属第一医院肿瘤外科 辽宁省沈阳市110001
马振海, 男,黑龙江省木兰县人，汉族.中国医科大学肿瘤外科学硕士，主要从事胃肠癌的基础及临床研究.
通讯作者：戴冬秋，110001，中国医科大学附属第一医院肿瘤外科.daidq63@163.com
电话: 024-23256666-6227
收稿日期: 2005-06-28 接受日期: 2005-07-18
彭丽华，杨云生，孙 刚，王巍峰，中国人民解放军总医院消化内科 北京市 100853
彭丽华,女，1972年生，北京市人，汉族，硕士，主治医师，主要从事消化系疾病的临床工作和胃肠功能性及动力疾病的研究.
通讯作者：杨云生，100853，北京市复兴路28号，解放军总医院消化内科. yangys@163bj.com
电话: 010-66939747
收稿日期: 2005-08-06 接受日期: 2005-08-25
Proteomic analysis of colonic mucosa by two-dimensional gelelectrophoresis in constipation-predominant irritable bowel syndrome
Li-Hua Peng, Yun-Sheng Yang,Gang Sun, Wei-Feng Wang
Li-Hua Peng, Yun-Sheng Yang, Gang Sun, Wei-Feng Wang, Department of Gastroenterology,General Hospital of Chinese PLA, Beijing 100853, China
Correspondence to:Yun-Sheng Yang, Department of Gastroenterology, General Hospital of Chinese PLA, 28 Fuxing Road, Beijing 100853, China. yangys@163bj.com
Received: 2005-08-06 Accepted: 2005-08-25
Abstract
AIM:To analyze the differential expression of proteome in colonic mucosa between patients with constipation-predominant irritable bowel syndrome (C-IBS) and the healthy controls.
METHODS: Two dimensional polyacrylamide gel elect-rophoresis (2-DE) technique and computer-assisted image analysis were used to separate the protein spots and analyze the differential expression of proteome in the colonic mucosa of the healthy controls and patients with C-IBS.
RESULTS: A total of 308 protein spots were identified in the av-erage gel of the healthy controls, and 238 in patients with C-IBS. A total of 178 protein spots were matched, and the mean matching rate was 74.49%. There were 18 protein spots that were significantly differentially expressed. Of those 18 protein spots, the expression of 3 spots were increased markedly, while 15 were decreased significantly ......
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