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应用抑制性消减杂交技术克隆乙型肝炎病毒DNAPTP1的反式调节基因
http://www.100md.com 高学松, 成 军, 甄 真, 郭 江, 张黎颖,
乙型肝炎病毒;DNA聚合酶;反式调节;抑制性消减杂交高学松, 成军, 甄真, 郭江­, 张黎颖, 陶明亮. 应用抑制性消
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     高学松,成军,郭江,张黎颖, 陶明亮北京地坛医院传染病研究所北京市 100011

    甄真,河北医科大学第三医院感染科河北省石家庄市 050051

    国家自然科学基金攻关项目, No. C03011402, No. C30070689

    军队九五科技攻关项目, No. 98D063

    军队回国留学人员启动基金项目, No. 98H038

    军队十五科技攻关青年基金项目, No. 01Q138

    军队十五科技攻关面上项目, No. 01MB135

    通讯作者:成军, 100011, 北京市东城区安外大街地坛公园13号, 北京地坛医院传染病研究所. cj@genetherapy.com.cn.

    电话: 010-64481639 传真: 010-64281540

    收稿日期:2005-03-01 接受日期:2005-07-05

    Cloning ofhepatitis B virus DNAPTP1 transactivating genes by suppression subtractivehybridization technique

    Xue-Song Gao, Jun Cheng, Zhen Zhen, Jiang Guo, Li-Ying Zhang, Ming-Liang Tao

    Xue-Song Gao, Jun Cheng, Jiang Guo, Li-Ying Zhang, Ming-Liang Tao, Institute of Infectious Diseases, Ditan Hospital, Beijing 100011, China

    Zhen Zhen, the Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei Province, China

    Supported by National Natural Science Foundation of China, No.C03011402, No.C30070689; Returned Scholarship of General Logistics Department of Chinese PLA, No.98H038; the Key Technology Research and Development Program of Chinese PLA during the 9th Five-Year Plan Period, No.98D063; the Key Technology Research and Development Program of Chinese PLA for Distinguished Young Scholars during the 10th Five-Year Plan Period, No.01Q138; and the Scientific Research Program of Chinese PLA during the 10th Five-Year Plan Period, No.01MB135

    Correspondence to: Dr. Jun Cheng, Institute of Infectious Diseases, Ditan Hospital, 13 Ditan Park, Anwai Street, Dongcheng District, Beijing 100011, China. cj@genetherapy.com.cn

    Received: 2005-03-01 Accepted:2005-07-05

    Abstract

    AIM: To clone and identify the new target genes transactivated by DNAPTP1 of the hepatitis B virus with the suppression subtractive hybridization technique and bioinformatics.

    METHODS: The mRNA was isolated from HepG2 cells transfected with pcDNA3 ......

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