【摘要】 目的 观察神经肽Y(neuropeptide Y，NPY)与去甲肾上腺素(NE)对心肌细胞生长、细胞内蛋白质合成的影响及二者的相互作用。 方法 进行原代乳鼠心肌细胞培养，以 3 H-亮氨酸掺入率的变化来反映NPY、NE对心肌细胞内蛋白质合成的影响。 结果 (1)NPY组、NE组 3 H-亮氨酸掺入率显著高于对照组(P<0.01);(2)NPY与NE合用可使心肌细胞 3 H-亮氨酸掺入率进一步升高，与二者单独使用组相比差异有非常显著性(P<0.01)。 结论 (1)NPY、NE均可促进心肌细胞内蛋白质的合成;(2)NPY与NE在促进心肌细胞内蛋白质合成过程中有明显的协同效应。

    【关键词】 神经肽Y;去甲肾上腺素;心肌细胞肥大

    The hypertrophic effects of NPY and NE on cardiomyocytes

    ZHOUYong，ZENG Chun-yu，Liu Guang-yao，et al.

    The466th Hospital of PLA，Beijing100089，China

    【Abstract】 Objective To investigate the effects of NPY and NE on cellular hypertrophy in cardiomyocytes，and conclusions were wished be uesful theoretic guides for clinical therapy.Methods Neonatal Wistar rat's cardiomy-ocytes were cultured first，then the effects of NPY，NE on cardiomyocytes growth were observed by measuring the changes of the 3 H-Leucine incorporation ratio in different concentrations and different times.Results (1)The 3 H-Leucine incorporation ratios were significantly higher in NPY and NE group than in control(P<0.01);(2)The 3 H-Leucine incorporation ratios of NPY+NE group were significantly higher than NPY and NE group(P<0.01).Con-clusion (1)NPY and NE could induce the hypertrophy of cardiomyocytes;(2)Being factors which were co-secreted from sympathetic nerves ......