【摘要】 目的 研究枸杞多糖对诱导人前列腺癌PC-3细胞凋亡及相关蛋白Bcl-2和Bax表达的影响。 方法 体外培养的人前列腺癌PC-3细胞经枸杞多糖作用后，采用MTT法测定细胞的增殖情况。采用TUNEL观察PC-3细胞凋亡的变化，用免疫组化法检测其Bcl-2和Bax蛋白的表达。 结果 一定剂量的枸杞多糖可明显抑制PC-3细胞的生长，并能诱导人前列腺癌PC-3细胞凋亡，凋亡率为41.5%，同时PC-3细胞Bcl-2/Bax蛋白比值显著下降，呈明显的剂量/效应关系。 结论 枸杞多糖能诱导人前列腺癌PC-3细胞的凋亡，其机制与调节人前列腺癌PC-3细胞Bcl-2和Bax蛋白表达有关。

    【关键词】 枸杞多糖;人前列腺癌细胞;细胞凋亡;Bcl-2;Bax

    Effect of lycium barbarum polysaccharides on human prostatic cancer cell line in-dependent of androgen apoptosis and apoptosis-related gene expression

    LI Zhuo-neng，LUO Qiong，YANG Ming-liang，et al.

    Department of Hygiene，School of Public Health，Wuhan University，Wuhan430071，China

    【Abstract】 Objective The aim of this study was to investigate the influence of Lycium barbarum polysaccharides(LBP)on human prostatic cancer cell line PC-3and its Bcl-2and Bax expression.Methods We cultured human prostate cancer PC-3cell in vitro and added the LBP to the medium at different concentrations.the MTT assay was used to measure the proliferation of PC-3cell.TUNEL was used to investigate the effects of LBP on PC-3cell apoptosis and immunohistochemical technique was used to detect the Bcl-2and the Bax expression.Results LBP could inhibit the growth of PC-3cell line.LBP can induce apoptosis and the apoptosis rate is41.5% ......