尿液中检测HIV-1抗体进行男同性恋HIV病毒感染筛查的研究
尿液,,男同性恋;酶联免疫吸附试验;,艾滋病病毒Ⅰ型,1材料与方法,2结果,3讨论,参考文献:
摘要:目的:用酶联免疫吸附试验(ELASA)进行男同性恋者尿液中艾滋病病毒Ⅰ型(HIV-1)抗体检测,并与血清学检测结果进行比较,探讨采用尿液检测HIV抗体进行特殊人群HIV感染筛查的可行性。方法:平行采集受检男同性恋者的外周静脉血及尿液标本,分别用血液和尿液ELISA法检测HIV抗体,阳性血清标本采用WB法确认。结果:检测109人,血清学检测结果3人阳性,并经WB实验确认为HIV病毒感染,尿液检测3人为阳性,与血清学实验对照,准确性为100%。结论:尿液检测HIV-1抗体结果可靠,可作HIV病毒感染的筛查。关键词:男同性恋;酶联免疫吸附试验; 艾滋病病毒Ⅰ型
Analysis of HIV-1 Antibody in Urine Samples for MSM AIDS Infection Test
SONG Dan,et al
(The Laboratory of Reproductive Center of Luohu Hospital,Guangdong Shenzhen 518001, China)
Abstract: Objective: To compare the consistency of result of detecting HIV-1 antibody in MSM urine samples and serum specimens by ELISA. Method:The grouped urine and serum samples of MSM were collected and HIV-1 antibodies in them were screened by urine and serum ELISA kits , respectively. The positive serum specimens were retested by western-blot test. Result:Of the 109 serum samples , 3 samples showed positive reaction; The results were confirmed by western-blot. Of the 109 urine samples , 3 showed positive reaction. The consistency of testing results was 100%.Conclusion: Urine specimen can be used to screen the HIV-1 antibody. ......
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