反相高效液相色谱法_

反相高效液相色谱法测定珍珠梅中槲皮素的含量

http://www.100md.com 《时珍国医国药》 2005年第9期

反相高效液相色谱法,,珍珠梅；反相高效液相色谱；槲皮素；含量,1仪器、试剂和药品,2方法与结果,3讨论,参考文献：

     摘要：目的：建立珍珠梅中槲皮素反相高效液相色谱法(RP-HPLC)含量测定方法。方法：采用RP-HPLC对槲皮素进行定量测定；采用YMC-pack ODS-A(4.6 mm×250 mm,5 μm)色谱柱，检测波长为370 nm。结果：色谱条件为流动相甲醇0.2%磷酸溶液(50∶50)，柱温为35℃，流速0.4 ml·min1。此法线性范围0.025～0.15 μg,r=0.999 9，精密度(RSD)为0.72%(n=5)；平均回收率为98.8%，RSD为0.69%(n=5)。结论：测定方法简便快捷、精密度高、准确性好，可以作为珍珠梅中黄酮的测定方法，以控制其原料的质量。

    关键词：珍珠梅；反相高效液相色谱；槲皮素；含量

    Determination of Quercetin in Sorbaria sorbifolia by RP-HPLC Assay

    ZHANG Xuewu1, GUAN Liping2, LI Tianzhu1

    (1.College of Medicine, Yanbian University, Yanji, Jilin, 133000, China; 2.College of Pharmacognosy, Yanbian University, Yanji, Jilin, 133000, China)

    Abstract:Objective:To establish an RP-HPLC method for the determination of quercetin in Sorbaria sorbifolia.Methods:The content of quercetin was determined by RP-HPLC. YMC-pack ODS-A(4.6 mm×250 mm,5 μm)column was used. The detection wavelength was set at 370 nm.Results:The mobile phase consisted of methanol-0.2% phosphatic acid (50∶50) with flow rate 0.4 ml·min1 and column temperature at 35℃. The method was linear within the range of 0.025～0.15 μg,(r=0.999 9). The method RSD was 0.72%(n=5). The average recovery of assay was 98.8% and RSD 1.45%(n=5).Conclusion:The method is simple, rapid and highly accurate and precise. It can be used for the quantitative determination of quercetinin Sorbaria sorbifolia. ......

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