12种西洋参中总皂苷及人参皂苷Rb1的测定比较
西洋参,,西洋参；总皂苷；人参皂苷Rb1；高效液相色谱法,1仪器与材料,2方法与结果,3讨论,参考文献：
摘要：目的：建立西洋参中人参皂苷Rb1含量测定的现代分析方法，并对12种西洋参中的总皂苷及人参皂苷Rb1进行测定，以观察不同产地对其含量变化的影响。方法：人参总皂苷含量测定采用比色法，人参皂苷Rb1含量测定采用高效液相色谱法。结果：人参皂苷Rb1含量在62～1 230 μg·ml-1之间呈良好线性，平均回收率为99.2%(RSD=1.69%)。12种西洋参中，有10种西洋参所含人参总皂苷均大于4%，人参皂苷Rb1含量大于1%。结论：该法操作简便，快速，重现性好，结果准确，可作为西洋参质量控制方法。关键词：西洋参；总皂苷；人参皂苷Rb1；高效液相色谱法
Determination and Comparison of the Contents of Total Ginsenoside and Ginsenoside Rb1 in Twelve Kinds of Panax quinquefolium. L
CHEN Junhui, XIE Mingyong*, ZHANG Zhiming, FU Zhihong, NIE Shaoping
(Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047, China)
Abstract:Objective:To develop a quick, convenient, modern analysis method for the determination of ginsenoside Rb1 in Panax quinquefolium L., to determine the contents of total ginsenoside and ginsenoside Rb1 of twelve samples and to compare the changes of the determined contents of samples origining from different regions.Methods:The contents of total ginsenoside were determined by UV-spectrophotometry, and the contents of ginsenoside Rb1 were determined by HPLC. Results:The calibration curve was linear over the concentration range of 62～123 0 μg·ml-1 for ginsenoside Rb1, the correlation coefficient was 0.999. The average recovery of the determination of ginsenoside Rb1 was 99.2% with the RSD of 1.69%. The contents of total ginsenoside of ten samples are more than 4%, while contents of ginsenoside Rb1 of ten samples are more than 1%.Conclusion:The developed method was found to be simple, fast, accurate and reliable for the determination of ginsenoside Rb1. ......
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