珠子参抑制小鼠肝癌及诱导细胞凋亡的研究
珠子参,,珠子参；肝癌H22细胞株；凋亡；肿瘤坏死因子,1材料,2方法与结果,3讨论,参考文献：
摘要：目的：观察珠子参对小鼠H22肝癌的抑制作用及其机理。方法：建立小鼠肝癌实体瘤和腹水瘤模型，观察珠子参对荷瘤小鼠抑瘤率和生命延长率，放免法(RIA)检测实体瘤小鼠血清中肿瘤坏死因子α(TNFα)含量，流式细胞仪技术(FCM)检测肿瘤细胞凋亡并进行周期分析。结果：珠子参能显著抑制肿瘤生长，抑瘤率达44.89%，且能明显延长荷瘤小鼠生命；珠子参组小鼠血清中TNFα浓度高于正常对照组，而比模型对照组低(P<0.05)；FCM分析显示，珠子参各组G0/G1期和S期细胞(%)减少，而G2/M期细胞和凋亡细胞(%)增加。结论：珠子参对H22肝癌小鼠具有良好的抑瘤作用，其作用机制可能与珠子参阻止G2/M期细胞转换而影响癌细胞在细胞周期中的进程，干扰S期DNA合成以及诱导小鼠肝癌细胞凋亡有关；此外珠子参能明显抑制荷瘤小鼠TNFα水平的异常增高，使其维持在一定水平而发挥抗肿瘤、调节机体免疫力等作用。关键词：珠子参；肝癌H22细胞株；凋亡；肿瘤坏死因子
Studies of PJ on Inhibition of Proliferation of H22 Cell in mice and Induction of Apoptosis
HU Wei， CHEN Tao
(Medical College of Three Gorges University, Yichang, Hubei 443002, China)
Abstract:Objective:To observe the anticancer action of Panax japlcus var (PJ) on mice liver cancer and its mechanism.Methods:Mouse models of the solid tumor of liver cancer and ascitic liver cancer were established. The inhibitory rate of solid tumor and the life prolongation rate were observed. The cell cycle and apoptosis of the cells were measured by Flow Cytometry (FCM).Radioimmunoassay (RIA) examined the content of TNFα in serum.Results: PJ could obviously inhibit the growth of tumor ......
您现在查看是摘要页，全文长 9538 字符。