HPLC 法测定β-榄香烯微乳剂中β-榄香烯的含量

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文章编号：(2004)06-0139-05,HPLC 法测定β-榄香烯微乳剂中β-榄香烯的含量

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    陈 闯，苏德森，王思玲

    (沈阳药科大学 药学院，辽宁 沈阳 110016)

    摘要：目的 建立测定β-榄香烯微乳剂中β-榄香烯含量的高效液相色谱法。方法 采用 Diamonsil C₁₈ 色谱 柱 (250 mm×4.6 mm，5 μm)，以甲醇-乙腈-水(体积比 40∶45∶15)为流动相，流速1.0 mL·min^-1，检测波长 230 nm，柱温为室温。结果 β-榄香烯在 0.2~1.0 g·L^-1 内呈良好的线性关系，相关系数 r = 0.999 9，精密度试验的 RSD 为 0.40%，方法平均回收率为 98.5%，其RSD为 0.69%(n=9)。结论 该方法为评价β-榄香烯微乳剂的质量提供了可靠保证。

    关键词：药剂学；微乳；高效液相色谱法；β-榄香烯

    中图分类号：R 917 文献标识码：A

    Determination of β-elemene in the micro- emulsion by HPLC

    CHEN Chuang, SU De-sen, WANG Si-ling

    (School of Pharmacy，Shenyang Pharmaceutical University，Shenyang 110016，China)

    Abstract: Objective To establish the analytical method for β-elemene in the micro-emulsion. Methods The content of β-elemene was determined by HPLC. Diamonsil-C₁₈(250 mm×4.6 mm，5μm)was used. The mobile phase consisted of methanol, acetonitrile and water (40: 45: 15，V: V: V). The flow rate was 1.0 mL·min^-1 and the UV detector was set at 230 nm. The column temperature was ambient temperature. Results The calibration curve was linear(r=0.999 9) within the concentration range of 0.2~1.0 g·L^-1 for β-elemene. The RSD of precision was 0.40%. The average recovery were 98.5% with RSD of 0.69%(n=9). Conclusions The method is suitable for the quality control the β-elemene micro-emulsion.

    Keywords: pharmaceutics；micro-emulsion；HPLC；β-elemene

    http://www.100md.com/html/DirDu/2005/10/19/78/30/40.htm

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