HPLC法测定氟尿嘧啶在小鼠血浆和组织中含量

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     及药物动力学研究

    郝秀华^1,2，程 刚¹，王 琳¹，于 杰¹，覃宇东¹，崔福德¹

    (1.沈阳药科大学药学院，沈阳 辽宁 110016；2.吉林大学药学院，长春 吉林130021)

    摘要:目的 建立高效液相色谱法测定小鼠血浆及组织样品中氟尿嘧啶(5-Fu)的浓度，并进行5-Fu在小鼠体内的药物动力学研究。方法 以5-溴尿嘧啶(5-Bru)为内标，血浆和组织样品经液-液萃取后，采用SHIMADZU高效液相色谱仪进行分析，色谱柱为Kromasil C₁₈ (4.6 mm×200 mm, 5 μm)，流动相为乙腈-水 (1︰99，V︰V)，检测波长为265 nm。结果 该法测定5-Fu在血浆及各组织中的平均回收率较高，日间、日内精密度符合有关规定。结论 方法操作简便、快速、适用于5-Fu在小鼠血浆、组织中的检测及药物动力学研究。

    关键词：药剂学；药物动力学；高效液相色谱法；氟尿嘧啶

    中图分类号：R917 文献标识码：A

    Determination of 5-Fu in mice plasma and tissues by high performance liquid chromatography and pharmacokinetic study

    HAO Xiu-hua^{1, 2}, CHENG Gang^1*, WANG Lin¹， YU Jie¹，QIN Yu-dong¹，CUI Fu-de¹

    (1.School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. School of Pharmacy, Jilin University, Changchun 130021, China)

    Abstract: Objective To develop a HPLC method for determining the concentration of 5-Fu in mice plasma and tissues, and study its pharmacokinetics. Methods 5-Bru was used as the internal standard and plasma and tissues samples were prepared based on a simple liquid-liquid extraction. The analytical column was Kromasil C₁₈ (4.6 mm×200 mm, 5 μm). The mobile phase was acetonitrile- water (1∶99, V∶V). The UV detective wavelength was 265 nm. Results The extraction recovery of 5-Fu in plasma and tissues was high, and the precision of inter-day and intra-day accorded with relevant regulation. Conclusion It appeared to be an accurate, convenient, and effective method, and is suitable for the detection of 5-Fu in mice plasma and tissues.

    Key words: pharmaceutics; pharmacokinetics; HPLC; 5-fluorouracil

    http://www.100md.com/html/DirDu/2005/10/19/78/32/36.htm

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