RP-HPLC法测定多西紫杉醇脂质体药物含量
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文章编号：(2003)03-0113-04,RP-HPLC法测定多西紫杉醇脂质体药物含量
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吴 琼，邓意辉，王绍宁，雷杰杰，李 毅
(沈阳药科大学药学院 ， 沈阳 110016 )
摘要: 目的 建立测定多西紫杉醇脂质体中药物含量方法。方法 采用Dikma ODS柱(4.6 mm×200 mm，5 mm，Dikma)；流动相：乙腈–水(50∶50，V∶V)；柱温：室温；流速：1 mL·min-1；检测波长：230 nm。结果 在本色谱条件下多西紫杉醇与辅料及溶剂峰分离良好，多西紫杉醇在5.05~40.4 mg·L-1内线性关系良好(r = 1.000 0，n = 5)，回收率在98.8% ~101.5%内，日内RSD及日间RSD均小于2% (n = 3)。结论 方法准确可靠、简单快速，可用于多西紫杉醇脂质体含量测定。
关键词 药剂学；脂质体；RP-HPLC；多西紫杉醇
中图分类号：R944 文献标识码：A
RP-HPLC determination of content of docetaxel liposome
WU Qiong, DENG Yi-hui, WANG Shao-ning, LEI Jie-jie, LI Yi
(School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China )
Abstract Objective: To establish a RP-HPLC method for the content of docetaxel in liposomes. Methods: A Dikma ODS column(4.6 mm×200 mm，5 mm)was used, the mobile phase was acetonitrile-water(50∶50), flow rate was 1 mL ·min-l and the maximum detection wavelength was 230 nm. Results: There is a good linear relation between the area and concentration of docetaxel in the range of 5.05~40.4 mg· L- l(r = 1.000 0, n = 5), the intra-day RSD and inter-day RSD were less than 2%, the average recovery was between 98.8%~101.5%. Conclusion: This method is simple, accurate, sensitive and applicable for determination of docetaxel in liposomes.
Key words: pharmaceutics；liposome；RP-HPLC ；docetaxel
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