HPLC法测定桂利嗪注射液的含量
http://www.100md.com
参见附件。
刘 莱1，王东凯1，徐松林1，程 楠2
(1.沈阳药科大学药学院，辽宁 沈阳 110016; 2.沈阳沃森药物研究所，辽宁 沈阳 110016)
摘要: 目的 建立HPLC法测定桂利嗪注射液的含量。方法 采用Diamonsil C18柱(250 mm× 4.6 mm, 5 μm )，以甲醇-0.2%三
乙醇胺-三乙胺(体积比85：15：0.03)并用磷酸调pH6.5作为流动相，流速1.0 mL·min-1，紫外检测波长为253 nm。结果 平均回
收率为99.67%，RSD为 0.63%(n＝9)，桂利嗪在质量浓度6～14 mg·L-1内与峰面积呈良好线性关系，r＝0.999 8。结论 该法可
用于测定桂利嗪注射液的含量，简便、准确。
关键词：药剂学；含量测定；高效液相色谱法；桂利嗪
中图分类号：R944 文献标识码：A
Determination of cinnarizine injection by HPLC
LIU Lai1，WANG Dong-Kai1，XU Song-lin1，CHENG Nan2
(1.School of Pharmacy，Shenyang Pharmaceutical University，Shenyang 110016，China； 2.Watson Pharmaceutical Institute of Shenyang，Shenyang 110016，China)
Abstract：Objective To establish an HPLC method for determination of cinnarizine injections. Methods A C18 column(Diamonsil 250 mm×4.6 mm, 5 μm ) was used to analyze cinnarizine. The mobile phase consisted of Methanol-0.2%triethanol amine-triethylamine(85:15:0.03)and pH6.5 adjusted by phosphoric acid. The flow rate was set to 1.0 mL·min-1. An UV detector with maximum wavelength 253 nm were adopted. Results The average recovery of 99.67% was obtained with a RSD of 0.63%(n=9). The linear range for cinnarizine was 6～14 mg·L-1 with the regression coefficient of 0.999 8. Conclusion Data obtained suggests that it is a simple and accurate method of assay for cinnarizine injections.
Key words：pharmaceutics; assay; HPLC；cinnarizine
刘 莱1，王东凯1，徐松林1，程 楠2
(1.沈阳药科大学药学院，辽宁 沈阳 110016; 2.沈阳沃森药物研究所，辽宁 沈阳 110016)
摘要: 目的 建立HPLC法测定桂利嗪注射液的含量。方法 采用Diamonsil C18柱(250 mm× 4.6 mm, 5 μm )，以甲醇-0.2%三
乙醇胺-三乙胺(体积比85：15：0.03)并用磷酸调pH6.5作为流动相，流速1.0 mL·min-1，紫外检测波长为253 nm。结果 平均回
收率为99.67%，RSD为 0.63%(n＝9)，桂利嗪在质量浓度6～14 mg·L-1内与峰面积呈良好线性关系，r＝0.999 8。结论 该法可
用于测定桂利嗪注射液的含量，简便、准确。
关键词：药剂学；含量测定；高效液相色谱法；桂利嗪
中图分类号：R944 文献标识码：A
Determination of cinnarizine injection by HPLC
LIU Lai1，WANG Dong-Kai1，XU Song-lin1，CHENG Nan2
(1.School of Pharmacy，Shenyang Pharmaceutical University，Shenyang 110016，China； 2.Watson Pharmaceutical Institute of Shenyang，Shenyang 110016，China)
Abstract：Objective To establish an HPLC method for determination of cinnarizine injections. Methods A C18 column(Diamonsil 250 mm×4.6 mm, 5 μm ) was used to analyze cinnarizine. The mobile phase consisted of Methanol-0.2%triethanol amine-triethylamine(85:15:0.03)and pH6.5 adjusted by phosphoric acid. The flow rate was set to 1.0 mL·min-1. An UV detector with maximum wavelength 253 nm were adopted. Results The average recovery of 99.67% was obtained with a RSD of 0.63%(n=9). The linear range for cinnarizine was 6～14 mg·L-1 with the regression coefficient of 0.999 8. Conclusion Data obtained suggests that it is a simple and accurate method of assay for cinnarizine injections.
Key words：pharmaceutics; assay; HPLC；cinnarizine
您现在查看是摘要介绍页，详见PDF附件。