尼群地平脂质体的制备及包封率的测定
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文章编号;(2005)04–0180–04,尼群地平脂质体的制备及包封率的测定
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李 喆 , 邓英杰 ，王秀敏
(沈阳药科大学 药学院，辽宁 沈阳 110016)
摘要：目的 制备尼群地平脂质体，测定脂质体的包封率。方法 采用单相溶液冻干法制备尼群地平脂质体，用超滤法分离脂质体和游离药物，紫外分光光度法测定其含药量和包封率，并考察了冻干产物的稳定性。结果 尼群地平脂质体的含药量为(0.60±0.02)g·L-1，包封率为(65.1±2.1)%，长期放置，包封率变化不大。结论 采用单相溶液冻干法制备尼群地平脂质体和紫外分光光度法测定脂质体包封率，方法可行。
关键词：药剂学；脂质体；冻干；尼群地平；包封率
中图分类号：R94 文献标识码：A
Preparation of nitrendipine liposomes and determination of encapsulation efficiency
LI Zhe, DENG Ying-jie, WANG Xiu-min
(School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China)
Abstract: Objective To prepare nitrendipine liposomes, determine its encapsulation efficiency by Ultraviolet spectrophotometry. Methods The nitrendipine liposomes were prepared by freeze-drying of monophase solution. The ultrafiltration method was used to separate the free nitrendipine from liposomes, ultraviolet spectrophotometry method was used to determine its drug content and encapsulation efficiency,and stability of liposomes was studied. Results The drug content of nitrendipine liposomes was (496±10.4) mg • L-1. The encapsulation efficiency was (50.25±0.62)%, and it did not change obviously after storage of 12 months. Conclusion It’s feasible to prepare nitrendipine liposomes by freeze-drying of monophase solution and determine its encapsulation efficiency by Ultraviolet spectrophotometry.
Key words: pharmaceutics; liposomes; freeze-drying; nitrendipine; encapsulation efficiency
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