胃癌细胞PTEN甲基化与其表达的相关性
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邓全军, 于皆平, 刘 芬, 齐元玲, 刘诗权,
PTEN基因;胃癌细胞;甲基化特异的PCR,邓全军,于皆平,刘芬,齐元玲,刘诗权,于红刚,通讯作者:,RelationshipbetweenPTENmethy
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邓全军, 于皆平, 刘芬, 齐元玲, 刘诗权, 于红刚, 武汉大学人民医院消化内科 湖北省武汉市 430060
国家自然科学基金资助项目, No.30300154
通讯作者: 于红刚, 430060, 湖北省武汉市, 武汉大学人民医院消化内科. smiledeng@126.com
电话: 027-88076874
收稿日期: 2005-09-10 接受日期: 2005-09-30
Relationship between PTEN methylation and its mRNA expression in human gastric cancer cells in vitro
Quan-Jun Deng, Jie-Ping Yu, Fen Liu, Yuan-Ling Qi, Shi-Quan Liu, Hong-Gang Yu
Quan-Jun Deng, Jie-Ping Yu, Fen Liu, Yuan-Ling Qi, Shi-Quan Liu, Hong-Gang Yu, Department of Gastroenterology, People’s Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
Supported by National Natural Science Foundation of China, No.30300154
Correspondence to: Hong-Gang Yu, Department of Gastroenterology, People’s Hospital of Wuhan University, Wuhan 430060,Hubei Province, China. smiledeng@126.com
Received: 2005-09-10 Accepted: 2005-09-30
Abstract
AIM: To investigate the relationship between the meth-ylation status of the 5’CpG island of PTEN promoter region and the mRNA expression of PTEN gene in gastric cancer cell lines in vitro.
METHODS: The methylation status of PTEN promoter region and expression of PTEN mRNA in gastric cancer cell lines SGC-7901 (moderately differentiated), BGC-823 (lowly differentiated), MGC-803 (lowly differentiated), HGC-27 (non-differentiated) were measured by methylation-specific polymerase chain reaction (MSP) and semi-quantitative reverse transc-ription PCR, respectively.
RESULTS: The methylation of PTEN gene in promoter region was found in HGC-27, MGC-803, and BGC-823 cells, but not in SGC-7901 ones. HGC-27 cells had the highest methylation level of PTEN gene (138.217 ± 7.898, P <0.01), then MGC-803 and BGC-823 cells, and no significant difference was found between MGC and BGC (P >0.05). SGC-7901 cells had the highest expression of PTEN mRNA (0.336 ± 0.079,P <0.01), then BGC-823, MGC-803 and HGC-27 (lowest, 0 ......
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