三氧化二砷诱导子宫颈癌HeLa细胞凋亡的初步研究
子宫颈癌,,三氧化二砷；,子宫颈癌；,HeLa细胞；细胞凋亡,1材料与方法,2结果,3讨论,参考文献：
摘要: 目的：研究三氧化二砷(As2O3)诱导子宫颈癌细胞系HeLa细胞凋亡，探讨As2O3对子宫颈癌的临床应用意义。方法：采用四甲基偶氮唑盐比色(MTT)法检测浓度分别为1.0、2.0、5.0、10.0 μmol/L的As2O3作用1、2、3、4 d后HeLa细胞的存活率，并进行形态学观察；采用细胞凋亡原位检测(TUNEL)法进行细胞凋亡的检测。结果：2.0 μmol/L As2O3处理HeLa细胞48 h后,可见细胞的存活率明显降低(P<0.05)，处理96 h后，细胞的存活率进一步降低(P<0.01)，经5.0 μmol/L As2O3处理的HeLa细胞24 h后就明显可见细胞的存活率降低；As2O3作用后，HeLa细胞具有明显的凋亡特征性改变；TUNEL法检测可发现HeLa细胞有DNA的断裂。结论：As2O3可以诱导子宫颈癌HeLa细胞凋亡，抑制细胞增殖，随着As2O3浓度增加，作用时间延长，效果越明显，其作用具有浓度和时间依赖性。关键词：三氧化二砷； 子宫颈癌； HeLa细胞；细胞凋亡
The primary study on programmed cell death of human uterin cervical cancer induced by arsenic trioxide
YANG Xu, Lalai, LU Xiaomei, et al
(Department of Pathology， Xinjiang Medical University, Urumqi 830054，China)
Abstract：Objective： To investigate the programmed cell death of human uterin cervical cancer induced by arsenic trioxide (As2O3) and research the arsenic trioxide significance of clinical application. Methods： Morphlogy investigation,MTT reduction assay and in situ cell death detection (TdTmediate Dutp nick labeling, TUNEL) were adopted. Results： The survival of HeLa cell line was inhibited by As2O3. And a typical apoptotic morphlogy also could be seen during the cells treated with As2O3. Moreover DNA fragmentation could be detected by TUNEL. Conclusion： As2O3 can induce programmed cell death of HeLa cells and inhibit the cellular proliferation. The effect shows time and concentration dependence. ......
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