维吾尔医__

异常黑胆质成熟剂与清除剂对H2O2诱导的淋巴细胞毒性的影响

http://www.100md.com 《新疆医科大学学报》 2005年第4期

维吾尔医,,维吾尔医；异常黑胆质成熟剂和清除剂；H2O2；,MTT；淋巴细胞,1材料与方法,2结果,3讨论,参考文献:

     摘要: 目的：观察异常黑胆质成熟剂和异常黑胆质清除剂对H2O2诱导的淋巴细胞毒性的作用。方法：采用健康人外周血中分离的淋巴细胞作为研究对象，用四氮甲基唑蓝法(MTT)检测淋巴细胞活性。结果：异常黑胆质成熟剂在100 μg/ml和50 000 μg/ml的浓度下，明显的拮抗H2O2 800 μmol/L H2O2对细胞生长的抑制。异常黑胆质清除剂对H2O2处理诱导的淋巴细胞的生长抑制未显示出明显作用。结论：异常黑胆质成熟剂能显著提高H2O2抑制的淋巴细胞的生存率。

    关键词：维吾尔医；异常黑胆质成熟剂和清除剂；H2O2； MTT；淋巴细胞

    Abnormal Savda Munziq and Mushil protection effect against H2O2induced DNA damage and cytotoxity in human lymphocyte

    LI Lin, JI Yanli, CHEN yan, et al

    (Department of Biochemistry, Xinjiang Medical University, Urumqi 830054,China)

    Abstract: Objective: To study the protective effect of Xinjiang Uighur Medicine Abnormal Savda Munziq and Mushil on hydrogen peroxide induced cytotoxity in human lymphocyte. Methods: The inhibitory effect of hydrogen peroxide on cell growth was determined using the tetrazolium dye colorimetric test (MTT test). Results: H2O2 (800μmol/L) suppressed the growth of human lymphocyte and preincubation of the cell with Abnormal Savda Munziq (100 μg/L and 50 000 μg/L) reduced the suppression. Treatment of Abnormal Savda Mushil (100 μg/L to 50 000 μg/L) did not display significant effect on cell growth supression of the H2O2 induced cell group. Conclusions: Abnormal Savda Munziq has a strong protective ability against cytotoxity caused by hydrogen peroxide. ......

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