细粒棘球蚴感染小鼠T淋巴细胞亚群的变化及意义
细粒,,细粒棘球蚴；CD4+淋巴细胞；CD8+淋巴细胞；流式细胞术,1材料与方法,2结果,3讨论,参考文献：
摘要： 目的： 探讨细粒棘球蚴病宿主体内T淋巴细胞亚群在免疫调节和发病中的作用。 方法： 对感染细粒棘球蚴的昆明种小鼠观察20周，在不同时间取脾制备细胞悬液，用流式细胞术(FCM)检测CD4+、CD8+淋巴细胞的百分率。 结果： 细粒棘球蚴感染后的小鼠，于2周时CD4+、CD8+淋巴细胞的百分率明显增高(P<0.01)，随后CD4+的百分率逐渐降低，CD8+的百分率逐渐增高，至感染16周后，CD4+的百分率较正常对照组下降(P<0.01)，CD8+的百分率较正常对照组升高(P<0.01)，CD4+/CD8+比值降低(P<0.01)。 结论： 感染细粒棘球蚴的小鼠早期以CD4+细胞反应为主，具有保护性免疫，感染后期逐渐以CD8+细胞为主，使机体呈免疫抑制状态，有利于细粒棘球蚴增殖。关键词： 细粒棘球蚴；CD4+淋巴细胞；CD8+淋巴细胞；流式细胞术
Changes in Tcell subpopulation in kunming miceinfected with Echinococcus granulosus
YU Fei, KANG Jinfeng, Iroc, et al
(Department of Pathogenic Biology, Xinjiang Medical University, Urumqi 830054, China)
Abstract: Objective: To explore the immunological regulation mechanism of mice infected with Echinococcus granulosus. Methods: The course of the infection was observed for 20 weeks. Spleen cells from mice infected with Echinococcus granulosus were collected in different period, CD4+ and CD8+ cell levels were determined by flow cytometre. Result: Two weeks after infection,the CD4+ and CD8+ cell levels showed a significant increase(P<0.01), then CD4+ cell decreased and CD8+ cell increased gradually. Since 16 weeks after infection,the percentage of CD4+ cell were higher than that of control group(P<0.01)and the percentage of CD8+ cell were lower than that of control group(P<0.01). There was a significant drop down of CD4+/CD8+ ratio since 16 weeks after infection(P<0.01). Conclusion: CD4+ cell is mainly response in the early stage of infection, CD4+ response is replaced by CD8+ response in later period of infection. CE host exhibits an immune inhibition response,which is very useful to hosts with Echinococcus granulosus infection. ......
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