Study on Determ i na tion of Polyphenols i n Honeysuckle Flower By

    M i crocolumn Hi gh Performance L iquid Chroma tography

    Hu Qiufen

    1, 2

    , Yang Guangyu

    2

    , Huang Zhangjie

    2

    , L i Haitao

    1

    , Yin J iayuan

    3 2

    1

    (Depart m ent of Chem istry, Yux i Teacher′ s College, Yuxi 653100)

    2

    (Depart m ent of Chem istry, Yunnan Univer sity, Kunm ing 650091)

    Abstract A microcolumn high perfor mance liquid chromat ography (HPLC) method for the determinati on of

    polyphenols ( chl orogenic acid, quercitrin2 32 glucoside, luteolin2 72 glucoside, luteolin2 72 galact oside,quercitrin, luteolin) in honeysuckle fl ower was studied . The polyphenols were extracted from the samp le by

    methanol with refluxing for 45 min . Then the polyphenolswas separated on aWaters Xterra

    T M

    RP18 (1 . 0 mm ×

    50 mm, 2 . 5μm) micr ocolumn with methanol and 1% acetic acid s oluti on gradient eluti on asmobile phase at ......