[摘要] 目的：以多孔β-磷酸三钙(β-TCP )生物陶瓷材料作为组织工程支架，以人牙周韧带细胞(PDLCs)作为种子细胞，探索构建组织工程化牙周膜的可行性。方法：将体外培养7 d的PDLCs和β-TCP的复合物，植入裸鼠背部一侧皮下，在对称部位植入单纯的β-TCP作为空白对照。术后4周、8周、12周分别取材，进行组织学检测。结果：实验组8周后支架材料部分吸收，可见未降解的支架材料孔洞内广泛分布着新生组织，其内可见小血管、腺体等结构；12周时支架材料大部分吸收，取而代之的是大量片状的新生结缔组织。空白组组织形成较少，支架材料降解与实验组相似。结论：体外培养的PDLCs与β-TCP复合后，能在体内增殖、分化，形成结缔组织，从而证明以β-TCP作为支架材料进行的牙周组织工程是可行的。

    [关键词] 组织工程化牙周膜 牙周韧带细胞 β-磷酸三钙

    A Study of Tissue Engineered Periodontal Ligament Based on Human Periodontal Ligament Cells and β-TCP.

    ZHANG Yi, WANG Yi-ning, ZHAO Yan, et al.

    Key Laboratory of Oral Biomedical Engineering (Wuhan University), Ministry of Education. Wuhan 430079

    [Abstract]Objective: To investigate the possibility of generate tissue engineered periodontal ligament by using porous β-TCP as scaffold and human periodontal ligament(PDL) cells as seed cell. Methods: PDLCs were seeded onto scaffolds and implanted subcutaneously in BALB/c-nu mice. The control group were implanted by β-TCP only. Formed tissues were harvested at 4,8,12 weeks after operation for histological analysis. Results: In the experimental group, histological analysis demonstrated obvious degradation of β-TCP and extensive new formation at 8 weeks after operation. The degradation of β-TCP was even obvious at 12 weeks after operation, and the cells on β-TCP formed sparse monolayers as well as the connected tissues. In the control group, only a small quantity of new formation could be seen. Conclusion: It is feasible to generate tissue engineered periodontal ligament by using porous β-TCP as scaffold and hPDLCs as seed cell. ......