【摘要】 目的 了解肿瘤坏死因子(tumor necrosis factor ，TNF)的致畸作用和胚胎毒性。方法 TNF设0.1、0.6和3.6μg/kg 3个剂量组，分别相当于人临床拟用剂量的3、18和108倍，于小鼠妊娠第6～15天连续皮下注射给药。每天1次。结果 溶剂对照组的活胎率(97%)、吸收胎率(1%)、死胎率(1%)和骨骼畸形率(3.5%)均在正常范围，与各给药组比较差异无显著性(P>0.05)。阳性对照组用环磷酰胺20mg/kg肌肉注射，外观畸形率达96%，表明本次实验结果可靠。结论 未发现TNF有胚胎毒性及致畸胎作用。

    【关键词】 肿瘤坏死因子；胚胎毒性；致畸胎作用

    Teratology studies with tumor necrosis factor in the mice

    LI Hai-yan, WANG Ai-ping, ZHANG Qing-lin,et al.

    Chinese Academy of Medicine Sciences and Peking Union Medical,Beijing 100050,China

    【Abstract】 Objective A teratology study of tumor necrosis factor was conducted in mice.Methods The TNF doses of 0.1, 0.6, 3.6μg/kg body weight/day in the mice were given by hypodermic injection on days 6～15 of gestation.Results The rate of viable fetuses, absorptions, dead fetuses and skeletal abnormality in vehicle control were 97%, 1%, 1% and 3.5%,were all in normal range, external abnormality of the positive control given cyclophosphamide at a dose of 20mg/kg body weight by intramuscular injection was 96%, showed the experiment was reliable.Conclusion The test indicated there was no evidence of embryotoxicity and teratogenicity in mice at any dose tested. ......