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    Gene expression of endothelin-1 in brain after cerebral ischemia and the effect of Yangyin Tongnao granule(养阴通脑颗粒) on it

    YANG Jie-hong, BIE Xiao-dong, DU Yue-guang, WAN Chen-xu, FAN Shou-yan, WAN Hai-tong

    Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, Zhejiang, China;

    OBJECTIVE：To observe the intervening effect of Yangyin Tongnao granule(养阴通脑颗粒)on gene expression of endothelin-1(ET-1) in brain after cerebral ischemia in rats, and to investigate the mechanism of its neuroprotective effects on cerebral ischemia injury. METHODS：In situ hybridization and method of image pattern analysis (MIPS) were used to detect the gene expression of ET-1 in cerebral cortex of ischemia side. RESULTS：ET-1 gene expressions were significantly increased in ischemic side in model group and Yangyin Tongnao granule compared with normal side. In ischemic side, ET-1 expression in cerebral cortex was lower in Yangyin Tongnao granule group than that in model group. CONCLUSION：Gene expression of ET-1 increases after cerebral ischemia. Yangyin Tongnao granule could reduce the gene expression of ET-1 in cerebral cortex of ischemia side, it plays a protective role on the brain injury. ......