胃癌高低发区差异基因型幽门螺杆菌菌株对人胃上皮细胞系GES-1 的损伤作用
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何红梅, 宫月华, 袁 媛
胃癌;幽门螺杆菌;差异基因型;胃黏膜上皮细胞;共培养;8-OHdG何红梅,宫月华,袁媛.胃癌高低发区差异基因型幽门螺杆菌菌株对人胃上皮细胞系GES-1的损伤作用.世界华人消化杂志2005;13(22)
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何红梅,宫月华, 袁媛, 中国医科大学附属第一医院肿瘤研究所第三研究室辽宁省沈阳市 110001
国家“十五”科技攻关资助项目,No.2004BA703B04-02
通讯作者:袁媛, 110001, 沈阳市和平区南京北街155号, 中国医科大学附属第一医院肿瘤研究所第三研究室.yyuan@mail.cmu.edu.cn
电话:024-23256666-6153 传真：024-22703576
收稿日期:2005-08-01 接受日期:2005-08-26
Damage effect of differentgenotype of Helicobacter pylori on human gastric epithelial cellline GES-1 in high- and low-risk areas of gastric cancer
Hong-Mei He, Yue-Hua Gong, Yuan Yuan
Hong-Mei He, Yue-Hua Gong, Yuan Yuan, the Third Department ofCancer Institute, the First Affiliated Hospital of China MedicalUniversity, Shenyang 110001, Liaoning Province, China
Supported by National Key Technologies Research and DevelopmentProgram of China during the 10th Five-Year Plan Period,No.2004BA703B04-2
Correspondence to:Professor Yuan Yuan, the Third Departmentof Cancer Institute, the First Affiliated Hospital of China MedicalUniversity, 155 North Nanjing Street, Heping District, Shenyang 110001,Liaoning Province, China. yyuan@mail.cmu.edu.cn
Received:2005-08-01 Accepted:2005-08-26
Abstract
AIM:To investigate the damage effect of the different genotypesof Helicobacter pylori on human gastric epit-helial cell line GES-1in high- and low-risk areas of ga-stric cancer, and to explore its relatedmechanism.
METHODS:H pylori were identified by hematoxylin-eosin(HE) staining, Gimsa staining, and Warthin-Starry silver staining. The DNAwas obtained by proteinase K and phenol-chloroform extraction method. ThecagA, vacAs1/s2, m1a, m1b, and m2 gene were amplified by polymerase chainreaction (PCR). The damage eff-ects of H pylori with differentialsub-genotypes and 2 other non-differential genotypes on GES-1 cells wereobserved by cell and H pylori co-culture. The expression of 8-OHdGin GES-1 cells was detected by S-P immun-ohistochemistry.
RESULTS: GES-1 cells were seriously damaged by H pylori ......
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