TGIF 对胃癌细胞株 SGC-7901 的维甲酸信号通路的影响
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丁莉利, 胡忠良, 郑长黎
胃癌;TG-相互作用因子;维甲酸信号通路.丁莉利,胡忠良,郑长黎.TGIF对胃癌细胞株SGC-7901的维甲酸信号通路的影响.世界华人消化杂志2005;13(22)2654-2657,TGIF对胃癌细
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丁莉利, 胡忠良, 郑长黎，中南大学湘雅医学院病理学教研室湖南省长沙市 410078
丁莉利, 女, 1975-09-12生, 湖南省益阳人, 病理学硕士,主要从事胃肠肿瘤的研究.
国家自然科学基金资助项目, No.30400208
通讯作者：郑长黎,410078, 湖南省长沙市湘雅路110号,中南大学湘雅医学院病理学教研室.changlizheng1125@yahoo.com.cn
电话: 0731-2650406 传真：0731-2650400
收稿日期：2005-09-28 接受日期：2005-10-25
Effects of TG-interacting factor on retinoic signaling pathway in gastric cancer cell line SGC-7901
Li-Li Ding, Zhong-Liang Hu, Chang-Li Zheng
Li-Li Ding, Zhong-Liang Hu, Chang-Li Zheng, Department of Pathology, Xiangya Medical College of Central South University, Changsha 410078, Hunan Province, China
Correspondence to:Chang-Li Zheng, Department of Pathology, Xiangya Medical College of Central South University, 110 Xiangya Road, Changsha 410078, Hunan Province,China. Changlizheng1125@yahoo.com.cn
Received:2005-09-28 Accepted:2005-10-25
Abstract
AIM:To investigate the influence of TG-interacting factor (TGIF) on the retinoid signaling pathway in gastr-ic cancer cell line SGC-7901 in vitro.
METHODS:Gastric cell line SGC-7901 were stably tra-nsfected with plasmid PcDNA3.1-TGIF and the highly expressing clone of TGIF was selected by Western blot. Then SGC-7901 cells were transiently transfected with TGIF oliganuleotides and the transfection efficien-cy were analyzed by reverse transcription polymerase chain reaction (PT-PCR). After treatment with 1 ?mol/L all trans-retinoic acid (ATRA), the proliferation of the cells was detected by MTT, and the apoptosis was measured by flow cytometry.
RESULTS:Before treatment with ATRA, the proliferat-ion and apoptosis of SGC-7901 cells were not significa-ntly different between PcDNA3.1-TGIF plasmids and TGIF oliganuleotides group. After treatment, the growth of TGIF transfected cells was more rapid than that of the cells transfected with empty vector and the parental cells (0.434±0.035 vs 0.360±0.014, 0.386±0 ......
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