时间分辨荧光免疫法与电化学发光法检测结直肠肿瘤标志 CEA 的比较
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张青云, 孙 丽, 张书耕, 王 琼
时间分辨荧光免疫分析;电化学发光免疫分析;癌胚抗原;结直肠癌,时间分辨荧光免疫法与电化学发光法检测结直肠肿瘤标志CEA的比较,张青云,孙丽,张书耕,王琼,通讯作者:,Comparisonbetw
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张青云, 北京大学临床肿瘤学院 北京肿瘤医院检验科 北京市肿瘤防治研究所免疫研究室 北京市 100036
孙丽, 锦州医学院生化教研室 辽宁省锦州市 121001
张书耕, 王琼, 北京大学临床肿瘤学院 北京肿瘤医院检验科 北京市 100036
通讯作者: 张青云, 100036, 北京大学临床肿瘤学院, 北京肿瘤医院检验科, 北京市肿瘤防治研究所免疫研究室. qingyzhang@btamail.net.cn
电话: 010-88115736
收稿日期: 2005-09-21 接受日期: 2005-11-12
Comparison between time-resolved fluorescence immunoassay and electro-chemiluminescence immunoassay for detection of serum carcinoembryonic antigen in colorectal carcinoma
Qing-Yun Zhang, Li Sun, Shu-Geng Zhang, Qiong Wang
Qing-Yun Zhang, Department of Medical Laboratory, Beijing Cancer Hospital, the School of Oncology, Pekjing University; Department of Immunology, Beijng Institute for Cancer Research, Beijing 100036, China
Li Sun, Department of Biochemistry, Jinzhou Medical College, Jinzhou 121001, Liaoning Province, China
Shu-Geng Zhang, Qiong Wang, Department of Medical Laboratory, Beijing Cancer Hospital, the School of Oncology, Pekjing University, Beijing 100036, China
Correspondence to: Dr. Qing-Yun Zhang, Department of Medical Laboratory, Beijing Cancer Hospital, the School of Oncology, Pekjing University; Department of Immunology, Beijng Institute for Cancer Research, Beijing 100036, China. qingyzhang@btamail.net.cn
Received: 2005-09-21 Accepted: 2005-11-12
Abstract
AIM: To evaluate the effect of time-resolved fluorescen-ce immunoassay (TRFIA) and electro-chemilumines-cence immunoassay (ECLIA) for detecting the serum level of carcinoembryonic antigen (CEA) and their influ-ence on the positive rate of serum CEA in patients with colorectal carcinoma.
METHODS: Serum CEA levels were detected with both TRFIA and ECLIA in 90 samples from patients with colorectal carcinoma (n = 30), non-colorectal car-cinoma (n = 30), and the healthy controls (n = 30). The data were analyzed with statistical software SPSS.
RESULTS: The level of CEA was significantly higher in the colorectal cancer group (TRFIA: 44 ......
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