大鼠胚胎神经干细胞的分离、培养和鉴定
胚胎,,大鼠;胚胎;神经干细胞,1材料和方法,2结果,3讨论,参考文献
【摘要】 目的：探讨胚胎神经干细胞的分离、培养和鉴定方法。方法：原代培养、培养细胞生长状况观察、组织化学鉴定、流式细胞仪进行细胞凋亡分析。结果：准确分离出了神经干细胞，了解其生长规律及细胞凋亡的情况。结论：用此方法分离胚胎神经干细胞实用、便捷和可行。【关键词】 大鼠;胚胎;神经干细胞
Extraction、Culture and Appraisal of the Rat Embryo Neural Stem Cells
JI Shengpu,WEI Huiping
Hebei North University，Zhangjiakou， 075000， China
【ABSTRACT】 Objective:To master extraction、culture and appraisal of the rat embryo neural stem cells initially by experiment,In this paper the growth rule and apoptosis of embryo neural stem cells were studied.Methods:the growth cycle and growth state for primary culture neural stem cells were observed carefully,and apoptosis was analyzed with histochemistry identitication and FCM(flow cytometry) as well.Results:investigated thororghly The neural stem cells were abstracted accurately, and their development cycle and apoptosis condition were investigated thoroughly.Conclusion:The result above can provide reliable evidence to obtain a great quantity of neural stem cells.
【KEY WORDS】 rat;embryo;neural stem cells
干细胞具有可再生为各种组织、器官的潜能 ......
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