肿瘤_____IFNα；DR4；DR5；肿瘤

IFNα对肿瘤细胞上TRAIL受体DR4和DR5表达的调节及意义

http://www.100md.com 《吉林医药医学学报》 2005年第1期

肿瘤,,IFNα；DR4；DR5；肿瘤,1材料与方法,2结果,3讨论,参考文献：

     摘要：目的研究IFNα对肿瘤细胞Colo205 TRAIL功能受体DR4和DR5表达的调节作用，以及IFNα刺激与肿瘤细胞对rTRAIL诱导凋亡敏感性的影响。方法Colo205细胞以IFNα刺激0、24、48和72 h；采用RTPCR方法检测DR4和DR5 基因表达；用间接免疫荧光染色结合流式细胞技术检测细胞表面DR4和DR5分子表达；采用annexinV和碘化丙锭双染色试剂盒检测细胞凋亡。结果IFNα刺激后，DR4和DR5 的mRNA在细胞表面的表达均有增加，刺激48 h，膜型DR4和DR5表达达到高峰值(16.7%和29.8%)；rTRAIL凋亡诱导效应在IFNα刺激48 h的Colo205细胞中最高。结论IFNα上调死亡受体DR4、DR5的表达可能是其促进rTRAIL 诱导肿瘤凋亡的重要机制。

    关键词：IFNα；DR4；DR5；肿瘤

    The significance of IFNα to the expression of trail receptor DR4 and DR5 in tumor cells

    LI Yan, CAO Yunxin, ZHANG Yun, HUANG Haiyan, JIN Baiquan

    (Department of immunity, Fourth Military Medical University, Xi′an, Shanxi Province, 710032,China)

    Abstract:ObjectiveStudying of regulating on IFNα to the expression of DR4 and DR5, and its affection to rTRAILinduced apoptosis in Colo205 tumor cells.MethodsColo205 cells were stimulated by IFNα for indicated time period (0,24,48 and 72 hours),the expression of DR4 and DR5 gene were determined by RTPCR assay. The expression of cell surfacebound DR4 and DR5 were determined by indirect fluorescence staining and flow cytometry analysis. An annexin V and PI staining kit were used to determined the apoptosis induced by rTRAIL. ResultsThe expression of mRNA and cell surfacebound DR4 andDR5 of Colo205 cells were up regulated by IFNα stimulation. The level surfacebound of DR4 andDR5 reached peak at 48 hours after IFNα treatment. The peak of apoptosis effect induced by rTRAIL also exerted in Colo205 cells stimulated by IFNα for 48 hours.ConclusionIFNα may accelerate the tumor cell apoptosis induced by rTRAIL by upregulating the expression of DR4 and DR5 of tumor cells. ......

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