医院感染__(期刊论文)

铜绿假单胞菌随机扩增DNA多态性基因分型研究

http://www.100md.com 《河北北方学院学报(医学版)》 2005年第3期

医院感染,,铜绿假单胞菌;随机扩增DNA多态性分析;基因分型;分子流行病学;医院感染;抗生素耐药谱,1材料与方法,2结果,3讨论,参考文献

     【摘要】目的：调查铜绿假单胞菌在临床各科中的流行情况，以期建立稳定的临床检测系统，在控制院内感染的流行病研究中发挥更大的作用。方法：用微量液体稀释法进行药敏检测，用RAPD技术进行基因分型。结果：耐药谱分6型，Ⅰ型占445%，Ⅱ型占185%，Ⅲ型占74%，Ⅳ型占74%，Ⅴ型占111%，Ⅵ型(不定型)占11%； RAPD分9型，A型占519%，B型占74%，C型占74%，D型占74%，E型占74%，F型占37%，G型占37%，H型占74%，I型占37%。结论：基因型A型、耐药谱Ⅰ型的铜绿假单胞菌是此次ICU医院感染暴发流行的菌株，其它型别为非流行相关菌株。 RAPD基因分型的灵敏性、特异性及可靠性优于耐药谱分型，在医院感染流行病学调查中具有较大的应用价值。铜绿假单胞菌对哌拉西林、头孢唑啉、头孢呋肟耐药率高；对喹诺酮类抗生素较敏感；对氨基糖甙类抗生素有一定的耐药性；亚胺培南的耐药率最低。

    【关键词】铜绿假单胞菌;随机扩增DNA多态性分析;基因分型;分子流行病学;医院感染;抗生素耐药谱

    Study on Genotypes of Pseudomonas Aeruginosa by Using Randomly Amplified Polymorphic DNA

    LIU Jinlu, WANG Lei, LI Caiqing

    The First Affiliated Hospital, Hebei North University, Zhangjiakou 075000 ,China

    【ABSTRACT】 Objective: By finding out the infection performance of Pseudomonas Aeruginosa (PA) in clinical practice of all departments to set up a stable clinical monitoring system of cross infection. Methods : Using RAPD technique for gene classifying and microdilution for drug- senstive test classifying. Results: 1.All the 27PA specimen collected were divided into 6 types according to different degrees of antibiotic drugresistance, of which Type Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ、took up 445、185%、74%、74%、111% and 11% respectively.2.By RAPD, all speoinen Genotype A 、B、C、D、E、F、G、H、I,occupying 519%、74%、、74%、74%、74%、37%、37%、74% and 37% respectively.Conclusion: Genotype A and Drug-Fast Spectrum Ⅰ in PA strains were the main causes of infection in ICU hospital, and other strains were not relevant to the infection. 2 the sensitivity , identification and reliability of RAPD classing obviously prevail over drug-fast spectrum, so it has considerable significance in the study of molecular epidemiology. 3 PA strains were highly resistant to Piperacillin ,Cefuroxime and Cefuroxime ; comparatively sensitive to Carbostyril antibiotics ; resistant to amimo sugar antibiotics in some degree and least resistant to Imipenem. ......

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