反相高效液相色谱法测定齐多夫定棕榈酸酯纳米乳剂药物含量
http://www.100md.com
文章编号:(2005)05–0311–05,反相高效液相色谱法测定齐多夫定棕榈酸酯纳米乳剂药物含量
参见附件。
魏守刚1，邓意辉1，隋志红1，高威2，刘宇2，陶闰红3
(1.沈阳药科大学 药学院，辽宁 沈阳110016；2.沈阳中海生物技术开发有限公司，辽宁 沈阳110016；3.沈阳医联药业有限公司，辽宁 沈阳 110004)
摘要：目的 建立测定齐多夫定棕榈酸酯纳米乳剂中药物含量的方法。方法 采用Betasil ODS柱(4.6 mm×200 mm, 5 μm)；流动相为甲醇-水(体积比为95:5)，流速为1.0 mL·min-1，检测波长为266 nm，柱温为室温。结果 在本试验条件下齐多夫定棕榈酸酯与辅料、溶剂及有关物质分离度均符合要求，在40~400 mg·L-1内呈良好的线性关系(r = 0.999 9, n = 7)，齐多夫定棕榈酸酯平均回收率为99.84%，RSD为0.35% (n = 3)。结论 本方法可用于齐多夫定棕榈酸酯纳米乳剂含量测定。
关键词：药剂学；齐多夫定棕榈酸酯；纳米乳剂；反相高效液相色谱法
中图分类号；R94 文献标识码：A
Determination of zidovudine palmitate in nanoemulsions by RP-HPLC
WEI Shou-gang1, DENG Yi-hui1, SUI Zhi-hong1, GAO Wei2, LIU Yu2, TAO Run-hong3
(1.School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 2.Shenyang Zhonghai Biological Technology Development Company Limited, Shenyang 110016, China; 3.Shenyang Yilian Pharmaceutical Company Limited, Shenyang 110004, China )
Abstract: Objective To establish a RP-HPLC method for the determination of zidovudine palmitate in nanoemulsions. Methods The separation was performed with a Betasil ODS column (4.6 mm×200 mm, 5 μm) at room temperature, and the mobile phase was consisted of methanol-water(95:5), the flow rate was 1 mL·min-1. Zidovudine palmitate was detected at 266nm. Results There was a good linear relationship between A and concentration over the range of 40~400 mg·L-1 (r=0.999 9, n=7). The average recovery was 99.84%. The RSD was 0.35%. Conclusion This method is applicable for the determination of zidovudine palmitate in nanoemulsions.
Key words: pharmaceutics; zidovudine palmitate; nanoemulsion; RP-HPLC
魏守刚1，邓意辉1，隋志红1，高威2，刘宇2，陶闰红3
(1.沈阳药科大学 药学院，辽宁 沈阳110016；2.沈阳中海生物技术开发有限公司，辽宁 沈阳110016；3.沈阳医联药业有限公司，辽宁 沈阳 110004)
摘要：目的 建立测定齐多夫定棕榈酸酯纳米乳剂中药物含量的方法。方法 采用Betasil ODS柱(4.6 mm×200 mm, 5 μm)；流动相为甲醇-水(体积比为95:5)，流速为1.0 mL·min-1，检测波长为266 nm，柱温为室温。结果 在本试验条件下齐多夫定棕榈酸酯与辅料、溶剂及有关物质分离度均符合要求，在40~400 mg·L-1内呈良好的线性关系(r = 0.999 9, n = 7)，齐多夫定棕榈酸酯平均回收率为99.84%，RSD为0.35% (n = 3)。结论 本方法可用于齐多夫定棕榈酸酯纳米乳剂含量测定。
关键词：药剂学；齐多夫定棕榈酸酯；纳米乳剂；反相高效液相色谱法
中图分类号；R94 文献标识码：A
Determination of zidovudine palmitate in nanoemulsions by RP-HPLC
WEI Shou-gang1, DENG Yi-hui1, SUI Zhi-hong1, GAO Wei2, LIU Yu2, TAO Run-hong3
(1.School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 2.Shenyang Zhonghai Biological Technology Development Company Limited, Shenyang 110016, China; 3.Shenyang Yilian Pharmaceutical Company Limited, Shenyang 110004, China )
Abstract: Objective To establish a RP-HPLC method for the determination of zidovudine palmitate in nanoemulsions. Methods The separation was performed with a Betasil ODS column (4.6 mm×200 mm, 5 μm) at room temperature, and the mobile phase was consisted of methanol-water(95:5), the flow rate was 1 mL·min-1. Zidovudine palmitate was detected at 266nm. Results There was a good linear relationship between A and concentration over the range of 40~400 mg·L-1 (r=0.999 9, n=7). The average recovery was 99.84%. The RSD was 0.35%. Conclusion This method is applicable for the determination of zidovudine palmitate in nanoemulsions.
Key words: pharmaceutics; zidovudine palmitate; nanoemulsion; RP-HPLC
您现在查看是摘要介绍页，详见PDF附件。