钼蓝法测定脂质体凝胶剂中磷脂的含量
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文章编号:(2005)05–0306–05,钼蓝法测定脂质体凝胶剂中磷脂的含量
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李宝齐，邓英杰，索绪斌
(沈阳药科大学 药学院，辽宁 沈阳 110016)
摘要： 目的 建立脂质体凝胶剂中磷脂的定量测定方法。方法 将样品用硝酸、高氯酸消化，调pH值后，加入钼蓝法显色剂，在830 nm处比色。并进行了线性、精密度、稳定性和加样回收率等项考察。结果 该方法线性方程为： A = 36.109ρ–0.0148，在4 ~ 20 mg·L-1内，线性关系良好(r = 0.999 4)，该方法精密度为：1.59%；显色后2 h比色结果稳定；加样回收率为：98.4% ~ 103.7%。结论 该方法适合于脂质体凝胶剂中磷脂含量的测定。
关键词: 药剂学；脂质体凝胶剂；磷脂；钼蓝法；含量测定
中图分类号: R94 文献标识码: A
Determinating contents of phospholipids in liposomal gel with molybdenum blue method
LI Bao qi, DENG Ying jie, SUO Xubin
(School of Pharmacy, Shenyang Pharmaceutical University ，Shenyang 110016，China)
Abstract: Objective To develop a method of determinating the contents of phospholipids in liposomal gel. Methods The sample was digested with nitric acid and perchloric acid, adjusted pH, added molybdenum blue reagent after adjusted pH,then colormetered at 830nm. In addition, the precision, stability, and the recovery of the method were also checked. Results The standard curve was A=36.109ρ-0.0148，with a good correlation in the concentration range of 4~20mg·L-1. The precision of the method was 1.59%(RSD), the absorption value was stable in 2h after color development. Conclusion This method is simple and is fit to determine of phospholipids contents in liposomal gel with good reproducibility.
Key words: pharmaceutics; liposomal gel; phospholipids; molybdenum blue method; content determination
李宝齐，邓英杰，索绪斌
(沈阳药科大学 药学院，辽宁 沈阳 110016)
摘要： 目的 建立脂质体凝胶剂中磷脂的定量测定方法。方法 将样品用硝酸、高氯酸消化，调pH值后，加入钼蓝法显色剂，在830 nm处比色。并进行了线性、精密度、稳定性和加样回收率等项考察。结果 该方法线性方程为： A = 36.109ρ–0.0148，在4 ~ 20 mg·L-1内，线性关系良好(r = 0.999 4)，该方法精密度为：1.59%；显色后2 h比色结果稳定；加样回收率为：98.4% ~ 103.7%。结论 该方法适合于脂质体凝胶剂中磷脂含量的测定。
关键词: 药剂学；脂质体凝胶剂；磷脂；钼蓝法；含量测定
中图分类号: R94 文献标识码: A
Determinating contents of phospholipids in liposomal gel with molybdenum blue method
LI Bao qi, DENG Ying jie, SUO Xubin
(School of Pharmacy, Shenyang Pharmaceutical University ，Shenyang 110016，China)
Abstract: Objective To develop a method of determinating the contents of phospholipids in liposomal gel. Methods The sample was digested with nitric acid and perchloric acid, adjusted pH, added molybdenum blue reagent after adjusted pH,then colormetered at 830nm. In addition, the precision, stability, and the recovery of the method were also checked. Results The standard curve was A=36.109ρ-0.0148，with a good correlation in the concentration range of 4~20mg·L-1. The precision of the method was 1.59%(RSD), the absorption value was stable in 2h after color development. Conclusion This method is simple and is fit to determine of phospholipids contents in liposomal gel with good reproducibility.
Key words: pharmaceutics; liposomal gel; phospholipids; molybdenum blue method; content determination
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