应用RNA干扰沉默血管内皮生长因子基因抑制HCT116细胞增殖的实验研究
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吕 伟, 张 超, 郭 红, 刘 伟, 郝迎学
RNA干扰;短发夹状RNA;血管内皮生长因子;细胞增殖,吕伟,张超,刘伟,郝迎学,郭红,通讯作者：,电话:,收稿日期:,接
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吕伟, 张超, 刘伟, 郝迎学, 中国人民解放军第三军医大学附属西南医院普外科 重庆市 400038
郭红, 中国人民解放军第三军医大学附属西南医院消化科 重庆市 400038
吕伟, 男, 1972-2-27 生, 江苏省金坛市人, 硕士研究生, 主治医师, 主要从事胃肠道肿瘤的外科及分子生物治疗研究.
重庆市自然科学基金资助项目, No. csfc-2005BB5291
通讯作者：张超, 400038, 重庆市沙坪坝区高滩岩正街30号, 第三军医大学附属西南医院普外科. zhangchao@163.com
电话: 023-68765274
收稿日期: 2005-10-14 接受日期: 2005-10-30
Suppression of HCT116 cell proliferation by silencing vascular endothelial growth factor gene with RNA interference
Wei Lv, Chao Zhang, Hong Guo, Wei Liu, Ying-Xue Hao
Wei Lv, Chao Zhang, Wei Liu, Ying-Xue Hao, Department of General Surgery, the Affiliated Southwest Hospital, the Third Military Medical University of Chinese PLA, Chongqing 400038, China
Hong Guo, Department of Gastroenterology, the Affiliated Southwest Hospital, the Third Military Medical University of Chinese PLA, Chongqing 400038, China
Supported by the Natural Science Foundation of Chongqing, No. csfc-2005BB529
Correspondence to: Chao Zhang, Department of General Surgery, the Affiliated Southwest Hospital, the Third Military Medical University of Chinese PLA, Chongqing 400038, China. zhangchao@163.com
Received: 2005-10-14 Accepted: 2005-10-30
Abstract
AIM: To observe whether the vascular endothelial growth factor (VEGF) short hairpin RNA (shRNA) expressed by recombinant plasmid Pavu6+27-VEGF siRNA in cells can effectively inhibit the proliferation of human colon carcinoma cell line HCT116.
METHODS: The constructed recombinant plasmid with the expression of shRNA was transfected into the human colon carcinoma cell line HCT116, and the empty plasmid (Pavu6+27) was transfected into the same cell line as controls. After screened with G418, flow cytometry was used to detect the distribution of the HCT116 cell cycles. The inhibitory rate of the cell growth was detected by MTT assay.
RESULTS: The recombinant Pavu6+27-VEGF siRNA effectively inhibited the proliferation of the cell line HCT116, and the inhibitory rates were significantly increased as compared with those in the controls at 24, 48, and 72 h (15 ......
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