幽门螺杆菌 VacA 重组蛋白对 SGC7901 胃癌细胞的作用
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段秀杰, 邵世和, 闻 平, 王文凯
幽门螺杆菌;空泡毒素;重组蛋白;胃癌细胞,幽门螺杆菌VacA重组蛋白对SGC7901胃癌细胞的作用,段秀杰,闻平,邵世和,王文凯,通讯作者:,EffectofrecombinantVacApro
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段秀杰, 闻平, 江苏大学附属医院检验科 江苏省镇江市 212001
邵世和, 江苏大学医学技术学院微生物教研室 江苏省镇江市 212001
王文凯, 北华大学研究生部 吉林省吉林市 132001
江苏省科技厅资助项目, No. BS2004021
江苏大学高级人才资助项目, No. JDG2004008
通讯作者: 邵世和, 212001, 江苏省镇江市, 江苏大学医学技术学院微生物教研室. ljxwl123@163.com
电话: 0511-2165735
收稿日期: 2005-10-22 接受日期: 2005-10-31
Effect of recombinant VacA protein of Helicobacter pylori on gastric cancer cell line SGC7901
Xiu-Jie Duan, Shi-He Shao, Ping Wen, Wen-Kai Wang
Xiu-Jie Duan, Ping Wen, Department of Clinical Laboratory, the Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China
Shi-He Shao, Department of Microbiology, Medical Technology College of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China
Wen-Kai Wang, Department of the Postgraduate, Beihua University, Jilin 132001, Jilin Province, China
Supported by the Project from Department of Science and Technology of Jiangsu Province, No. BS2004021, and the Fund for Senior Talent of Jiangsu University, No. JDG2004008
Correspondence to: Shi-He Shao, Department of Microbiology, Medical Technology College of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China. ljxwl123@163.com
Received: 2005-10-22 Accepted: 2005-10-31
Abstract
AIM: To explore the effect of the recombinant protein expressed byHelicobacter pylori (H pylori) VacA gene in E.coli on the gastric cancer cell line SGC7901.
METHODS: The engineered bacteria pET32a-VacA-E.coli BL21 (DE3) was cultured and analyzed. The isopropyl-β-D-thiogalactopyranoside (IPTG) was used to induce the expression of the recombinant protein. Then the purified protein was added into the gastric cancer cells, and the growth and morphological changes of the cell were observed by the inverted microscope and electron microscope.
RESULTS: The proliferation of the gastric cancer cells was inhibited after the recombinant protein was added ......
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